ISSN:
1572-9699
Keywords:
Methylophilus methylotrophus
;
NADH oxidation
;
Methanol oxidase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Previous reports that whole cells of Methylophilus methylotrophus oxidase exogenous NADH have been investigated. Essentially identical rates of oxygen consumption were observed following the addition of methanol or NADH to whole cells. Both activities were inhibited by EDTA and hydroxylamine, but not by HQNO, and exhibited similar pH optima. Analyses of the reaction stoichiometry with NADH as substrate showed that the expected amount of oxygen was consumed, but also revealed acidification (instead of alkalinisation) and no oxidation of NADH. Further studies showed that commerical NADH is contaminated with ethanol which is oxidised to acetic acid by the low specificity methanol oxidase system present in this organism. The oxidation of exogenous NADH by whole cells of M. methylotrophus reported previously is therefore spurious.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00393466
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