ISSN:
0736-0266
Keywords:
Autoradiography
;
Microautoradiography
;
Bone
;
Technetium
;
Bone-scanning agents
;
Diphosphonate
;
Life and Medical Sciences
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Technetium-99m methylene diphosphonate ([99mTc]MDP) is the most widely used bone-scanning agent today. In order to determine the precise bone locus of 99mTc corresponding to the delayed bone scan image, and to test the potential clinical use of this agent in enhancing the information obtained by bone scan, we employed [99mTc]MDP in this microautoradiographic study of normal bone and bone repairative tissue. Four white rabbits underwent operations in which two 1.5-mm drill holes were created in the subtrochanteric regions of both of their femora. An additional four white rabbits underwent sham operations of their femora, in which neither drilling nor periosteal injury occurred. Two rabbits were controls and did not undergo operation. After 7 days, the first two groups of rabbits were injected with [99mTc]MDP and bone scanned 2 h later. After the scans were completed, all three groups of animals were killed and their femora histologically processed for microautoradiography and routine histopathology. In the two groups that were injected with [99mTc]MDP, all bones showed the isotope to be clearly localized along mineralization fronts. The isotope was occasionally found in the substance of the osteoid, but was absent from the cytoplasm and nuclei of osteoblasts and osteocytes. Osteocytic lacunae showed the presence of isotope at their borders, but no identifiable pattern of isotope uptake was noted. Neither osteoclasts nor Howships lacunae showed isotope uptake. The animals that received drill holes showed corresponding areas of uptake by bone scan, whereas those that did not receive drill holes showed no bone scan activity at these sites. The histological findings were qualitatively similar in the bones from both groups of animals, but were quantitatively greater in the bones undergoing repair. The uninjected animals showed no positive chemography on their slides, indicating that the histological fixing and embedding solutions did not introduce artifacts into the technique. Skeletal scintigraphy has been shown to be a valuable clinical tool in the assessment of pathological conditions of bone. Through the use of microautoradiography, a correlation between the information gained by a bone scan and that of a bone biopsy or excised bony lesion can be made. This may aid in the assessment of the biological behavior of tumors or other pathological conditions of bone, as well as the precision of the bone scan in defining or localizing an osseous lesion.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jor.1100040206
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