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  • Electronic Resource  (2)
  • 1985-1989  (2)
  • Cellular polarity  (1)
  • Glycolytic intermediates  (1)
Material
  • Electronic Resource  (2)
Years
  • 1985-1989  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of biometeorology 32 (1988), S. 52-55 
    ISSN: 1432-1254
    Keywords: Seasonal variation ; Cold exposure, acute ; Glycolytic intermediates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract Seven male students were studied to observe the effects of acute cold exposure (at 10°C for 60 min) on erythrocyte concentrations of glycolytic intermediates in summer and in winter. The subjects shivered slightly but frankly in both experiments. Significant decreases were observed in the concentrations of pyruvate and lactate during body cooling in summer, but not in winter. The lactate concentration remained significantly reduced 15 min after cold exposure. After 60 min of cold exposure in summer, a negative crossover point appeared to exist between phosphoenolpyruvate and pyruvate and erythrocyte pyruvate kinase activity showed a significant decrease. No seasonal difference was observed in the initial control values of the intermediates measured. From these results and the fact that glucose, pyruvate and lactate are evenly distributed between erythrocytes and plasma, it is likely that erythrocytes and skeletal muscles need less fuel substrate, glucose during cold exposure in winter than in summer, suggesting that an increased economy of energy for homeostasis is achieved.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Thyroid follicle ; Suspension culture ; Cellular polarity ; Tight junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Porcine thyroid follicles, when isolated by enzymatic digestion and suspended in Eagle's MEM containing 10% fetal calf serum, undergo inversion of cellular polarity. After isolation, the strands for the tight junctions (zonulae occludentes) between follicle cells begin to move towards the side of the medium and gather at this side of the lateral plasma membrane during 24 h of incubation. Around this time, microvilli of many follicular cells protrude into the culture-medium. The elements of the Golgi apparatus are located at the luminal as well as the culture-medium side of the cytoplasm, and also at the lateral side of the nucleus after 24 h of suspension culture, and by 94 h of incubation almost all elements of this organelle, as well as lysosomes and the central cilium have migrated to the side of the medium. The migration of the zonulae occludentes is considered to be the initial change in the reversal of the polarity of this cell.
    Type of Medium: Electronic Resource
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