ISSN:
1432-2242
Keywords:
Triticum aestivum
;
SCEs
;
Auxin
;
Cytokinin
;
Tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In order to know the mutagenic effects of synthetic auxins (NAA, 2,4-D, and 2,4,5-T) and a cytokinin (kinetin) in vitro, sister chromatid exchanges (SCEs) were analyzed in cultured cells of a hexaploid wheat (Triticum aestivum L.). In the MS medium supplemented with 2.0 mg/l 2,4-D, the mean number of SCEs per cell was 15.2, and per pg of DNA, 0.42. No significant effect was found in the treatments of NAA or 2,4-D at concentrations of 0.5–10.0 mg/l, whereas more than 2.0 mg/l of 2,4,5-T induced dramatic increases of SCEs. Kinetin itself had no significant effect on SCE induction, but there was a tendency that SCEs induced by 2,4,5-T were suppressed by kinetin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290836
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