ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A structural component of lipopolysaccharide, 3-deoxy-d-manno-2-octulosonic acid (KDO), was determined in Legionella pneumophila by gas-liquid chromatography (GLC) and confirmed by gas chromatography/mass spectrometry (GC/MS), using isolated lipopolysaccharide (LPS) and whole cells of L. pneumophila. KDO was liberated from cellular components by acid hydrolysis, and the dried hydrolysate was derivatized to form volatile and stable compounds for analysis by GLC. The GLC procedure overcomes some deficiencies of the conventional colorimetric thiobarbituric acid (TBA) method, and has adequate resolution to determine KDO in hydrolyzed whole cells without interference from other cellular components. The KDO content of the LPS was determined to be 0.53% which is in agreement with 0.65% previously found by the TBA method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1987.tb02289.x
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