ISSN:
1432-1424
Keywords:
Membrane-transport
;
fluorescence
;
chlortetracycline
;
X537 A
;
alamethicin
;
ionophore
;
liposome
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Conceptual advances in the field of membrane transport have, in the main, utilized artificial membranes, both planar and vesicular. Systems of biological interest,viz., cells and organelles, resemble vesicles in size and geometry. Methods are, therefore, required to extend the results obtained with planar membranes to liposome systems. In this report we present an analysis of a fluorescence technique, using the divalent cation probe chlortetracycline, in small, unilamellar vesicles, for the study of divalent cation fluxes. An ion carrier (X537 A) and a pore former (alamethicin) have been studied. The rate of rise of fluorescence signal and the transmembrane ion gradient have been related to transmembrane current and potential, respectively. A second power dependence of ion conduction-including the electrically silent portion thereof — on X537 A concentration, has been observed. An exponential dependence of “current” on “transmembrane potential” in the case of alamethicin is also confirmed. Possible errors in the technique are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870464
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