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  • Electronic Resource  (8)
  • 1980-1984  (8)
  • 1
    Electronic Resource
    Electronic Resource
    Replicon size and mean rate of DNA synthesis in rye (Secale cereale L. cv. Petkus Spring) (1982)
    Springer
    Chromosoma 86 (1982), S. 115-122 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chromosomal DNA replication was investigated in root meristem cells of Secale cereale L. cv. Petkus Spring using DNA fibre auto-radiography. At 23 ° the mean rate of replication, per single replicon fork, was 12.1 μm/h. Replicon size was between 20–25 μm. These results are compared with corresponding measurements for other angiosperm species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330733
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleolar organizers and fibrillar centres in Triticum aestivum L., cv. Chinese spring (1982)
    Springer
    Chromosoma 87 (1982), S. 447-459 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Serial sectioning of wheat roots prepared for electron microscopy was used to count the number of fibrillar centres per nucleus and nucleolus and to calculate their sizes. After growth at 35 ° C for two days the nucleoli became segregated and a one to one relationship was evident between chromosomal nucleolar organizers and fibrillar centres. This was confirmed using an aneuploid line carrying an additional pair of organizers. Quantitative studies showed that the fibrillar centres occupied a volume 0.24% of the total chromatin reticulum. From this it was calculated that only about 1/3 of the fibrillar centre material was likely to be nucleolar organizer chromatin. The other material was considered to be the protein revealed in silver staining studies. The importance of this was shown by its constant ratio to the size of all nucleoli in a given nucleus. — Evidence was found for the movement and fusion of organizer flanking regions during growth at 35 ° C. The number of junctions between chromatin and nucleolar organizers drops by about half giving one per organizer after segregation, and serial sectioning demonstrated such junctions in close proximity, an arrangement suggestive of incipient fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333466
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  • 3
    Electronic Resource
    Electronic Resource
    “Cuckoo” Aegilops addition chromosome in wheat ensures its transmission by causing chromosome breaks in meiospores lacking it (1984)
    Springer
    Chromosoma 90 (1984), S. 84-88 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In monosomic additions of Aegilops sharonensis to Chinese Spring wheat (2n=42 wheat chromosomes + 1 homoeologous group 4 Aegilops chromosome known as 4S1), probably all functional gametes carried one Aegilops chromosome. Such preferential transmission is unusual in monosomic alien additions. Male and female meiosis seemed usually normal, but about 75% of embryo sacs and 28% of pollen grains were visibly abnormal near anthesis. Before the first gametophyte mitosis, up to about 13% of megaspores and pollen grains showed abnormalities usual in wheat aneuploids. However, in first mitosis, 50% of megaspores at metaphase and anaphase and 41% of pollen grains at anaphase and telophase contained acentric chromosome (or chromatid) segments of various sizes, up to about 32 in a cell, which were separated from the rest of the chromosome (or chromatid) by a gap or a thin Feulgen-positive thread. Such separated segments (SSs) are not normally seen in wheat and its aneuploids. The data indicat that alien and wheat chromosomes interact in meiocytes so that meiospores with the alien chromosome develop into normal gametophytes, but meiospores lacking the alien chromosome have SSs at first mitosis by whose loss or unequal distribution between daughter nuclei sterilizing deficiencies arise. Thus only gametophytes with the alien chromosome are competent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352282
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  • 4
    Electronic Resource
    Electronic Resource
    Somatic deletion and redistribution of telomeric heterochromatin in the genus Secale and in Triticale (1983)
    Springer
    Chromosoma 88 (1983), S. 293-298 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract C-banding analysis of populations of Secale kuprijanovii L., S. cereale L., and x Triticosecale Wittmack established that Secale chromosomes that were modified by the loss of a telomeric C-band arose spontaneously by breakage in somatic tissue and could be stabilized and maintained over at least two generations. In S. cereale approximately double-sized C-bands were seen on every arm that originally contained a C-band except 1RS, 2RS, 3RS, and 7RS. One plant was homomorphic for an amplified band on 3RL which was stable over two generations. In the tetraploid triticale analyzed, an amplified telomeric C-band was found on 5RS and was stable in the homomorphic condition for two generations. Even though Secale chromosomes with deleted or amplified telomeric C-bands can arise spontaneously in the somatic and germ tissue of Secale species and triticale, they are not common. The conditions required for their formation and stabilization within a population are not known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292906
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  • 5
    Electronic Resource
    Electronic Resource
    Development and characterisation of a line of bread wheat, Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 327-334 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Triticum ; Polyacrylamide gel-electrophoresis ; Light and electron microscopy ; Chromosome deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary About 360 offspring of a tri-parental cross were screened by gel electrophoresis and unexpectedly one of them did not contain chromosome 1B ω-gliadins derived from either of the primary parents. A line disomic for the ω-gliadin null was developed from the surviving embryo half of the unique grain. Two dimensional electrophoresis revealed that all the storage protein genes at Gli-B1, coding for γ-gliadins, β-gliadins and low-molecular-weight subunits of glutenin as well as the ω-gliadin, were not expressed. The nuclei of dividing root-tip cells were shown by light microscopy to lack the normal short-arm satellites of chromosome 1B, indicating that the genes for the missing storage proteins had been lost through a terminal deletion. Using a radioactive ribosomal RNA probe, the deficient 1B chromosomes were shown to contain ribosomal RNA genes demonstrating that at least two-thirds of the short arm was still present. Examination of serial sections of chromosome 1B at metaphase by low-power electron microscopy showed that the point of scission of this chromosome was within the secondary constriction where the ribosomal RNA genes are located. The Gli-B1 locus must therefore be carried on the short-arm satellite. Transmission of the deficient chromosome from female gametes to progeny was normal (i.e., about 50%) but from pollen it was poor (8.8%). Recombination mapping indicated that the distance from the ribosomal RNA genes (Nor1) to Glu-B1 was 22 cM, equivalent to 13 cM from Nor1 to the centromere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267886
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  • 6
    Electronic Resource
    Electronic Resource
    The karyotype of tuleen 346 barley (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 53-58 
    Details
    ISSN: 1432-2242
    Keywords: Barley ; Interchange ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tuleen 346 barley is a triple homozygote for 3 unequal interchanges, T1-5v, T2-6y and T3-7d, which were induced independently in the variety, ‘Bonus’. It has great potential value in studying differences in behaviour and position among chromosomes within the same cell in barley and its hybrids since at least 5 and usually all 7 chromosome types can be identified in Feulgen preparations, compared with only 3 types in normal barley. Measurement of chromosome arm and satellite lengths in 10 haploid root-tip metaphases showed that in all comparisons except of the longest with the next longest, the 7 chromosome types were distinct in total length (P〈0.001–0.05) and in 8 of the 10 cells, the longest chromosome had a smaller arm ratio than the next longest. In these preparations, each of the 5 shortest chromosomes was easily identifiable by size and morphology. The longest chromosome was about 2.2 times as long as the shortest. C-banding showed that each of the 7 chromosome types had a clearly unique band pattern. The C-band patterns of interchange chromosomes confirmed and increased the precision of previously published breakpoint locations, viz. the short arms of chromosomes 1, 2, 3 and 7 and the long arms of chromosomes 5 and 6.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276283
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  • 7
    Electronic Resource
    Electronic Resource
    Preferential C-banding of wheat or rye chromosomes (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 227-233 
    Details
    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Rye ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different stains, wheat chromosomes could be distinguished from rye chromosomes by preferential staining. C-bands of rye chromosomes were preferentially stained with Giemsa while those of wheat chromosomes were preferentially stained with either Leishman or Wright stain. Preferential staining aids the identification of wheat and rye chromosomes and chromosome segments and in particular the recognition of wheat/rye chromosome substitutions and translocations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304000
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  • 8
    Electronic Resource
    Electronic Resource
    Purification of lumicolchicine for use in studies of plant development (1980)
    Springer
    Protoplasma 103 (1980), S. 105-114 
    Details
    ISSN: 1615-6102
    Keywords: Colchicine ; Lumicolchicine ; Microtubules ; Mitosis ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lumicolchicine was purified by preparative thin-layer chromatography. Tests for purity were ultraviolet absorption spectrophotometry, analytical thin-layer chromatography, and a bioassay using wheat roots. Wheat roots treated for 3 days with 10−3 M lumicolchicine showed no c-mitosis, but had reduced growth compared with controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276669
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