ISSN:
1432-072X
Keywords:
Nickel
;
Carbon monoxide oxidation
;
Clostridium pasteurianum
;
Nitrilotriacetate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Formation of carbon monoxide dehydrogenase in growing Clostridium pasteurianum was found to be dependent on trace nickel present as contaminant in the growth medium. The evidence is: i) Synthesis of the enzyme was increased, when NiCl2 (0.1 μM) was added to the medium; ii) Synthesis of the enzyme was almost completely inhibited when the cells were grown in the presence of nitrilotriacetate (0.1 mM) or of other chelating agents, which inhibited the uptake of trace nickel from the medium; iii) Inhibition of enzyme synthesis by the chelators could be specifically overcome by supplementing the medium with nickel (1μM).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00408054
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