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  • Electronic Resource  (4)
  • RAPD  (2)
  • (Mitochondrial membrane)  (1)
  • Allosteric model Binding Gating Neuronal nicotinic receptors Single channel  (1)
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  • Electronic Resource  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of phospholipid-ATPase complex interaction by the adenine nucleotide carrier
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 637 (1981), S. 400-407 
    Details
    ISSN: 0005-2728
    Keywords: (Mitochondrial membrane) ; ATPase ; Adenine nucleotide carrier ; Membrane reconstitution ; Oxidative phosphorylation ; Phospholipid
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(81)90044-X
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gating of α3β4 neuronal nicotinic receptor can be controlled by the loop M2-M3 of both α3 and β4 subunits (1999)
    Springer
    Pflügers Archiv 439 (1999), S. 86-92 
    Details
    ISSN: 1432-2013
    Keywords: Allosteric model Binding Gating Neuronal nicotinic receptors Single channel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Previous studies have shown that the gating mechanism of α3β4 neuronal nicotinic receptors is affected by a residue in the middle of the M2-M3 loop of the β4 subunit. We have extended the study of the same location to the α3 subunit. Bovine α3β4 receptors were mutated in position 268, substituting the residue present in wild-type receptors, i.e. leucine in α3 and asparagine in β4, for an aspartate. Wild-type and mutated α3 and β4 subunits were combined to form four different receptors. We have measured macroscopic currents in Xenopus oocytes elicited by nicotine, and related them to surface receptor expression measured with an epibatidine-binding essay. We also obtained single-channel recordings of the receptors to study their kinetic behaviour. The results were analysed in terms of an allosteric model with three states. We found that the effect of the mutation in the α3 subunit on the gating of the receptor was similar to the corresponding mutation in the β4 subunit. The effect when both subunits were mutated was additive, suggesting that the contribution of each subunit to the gating mechanism is independent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900143
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    RAPD fingerprints for identification and for taxonomic studies of elite poplar (Populus spp.) clones (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 54-59 
    Details
    ISSN: 1432-2242
    Keywords: Poplar ; Populus ; PCR ; RAPD ; Arbitrary primers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RAPD (Random Amplified Polymorphic DNA) fingerprints have recently been used to estimate genetic and taxonomic relationships in plants. In this study RAPD analysis was performed on 32 clones belonging to different species of the genus Populus. Of these, 25 clones are registered in several countries for commercial use and, altogether, cover almost 50% of the worlds cultivated poplars. DNA was prepared from leaves and amplified by PCR using random oligonucleotide primers. Amplification products were separated by agarose-gel electrophoresis to reveal band polymorphisms. Four primers out of the 18 tested, were selected on the basis of the number and frequency of the polymorphisms produced. With these a total of 120 different DNA bands were reproducibly obtained, 92% of which were polymorphic. The polymorphisms were scored and used in band-sharing analyses to identify genetic relationships. With a few but interesting exceptions, these are consistent with the present taxonomy of the genus Populus and with the known predigrees of cultivated poplars. Moreover, the results show that RAPD analysis allows one to discriminate among all tested clones and can, therefore, be recommended as a convenient tool to defend plant breeders rights.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223744
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories (1997)
    Springer
    Molecular breeding 3 (1997), S. 381-390 
    Details
    ISSN: 1572-9788
    Keywords: DNA markers ; RAPD ; AFLP ; SSR ; microsatellite ; network ; reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and comparison of the data are possible. This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length polymorphism (AFLP) and sequence-tagged microsatellites (SSR). For each technique, an optimal system was chosen, which had been standardised and routinely used by one laboratory. This system (genetic screening package) was distributed to different participating laboratories in the network and the results obtained compared with those of the original sender. Different experiences were gained in this exchange experiment with the different techniques. RAPDs proved difficult to reproduce. For AFLPs, a single-band difference was observed in one track, whilst SSR alleles were amplified by all laboratories, but small differences in their sizing were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009612517139
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    Paper (German National Licenses)
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