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  • Electronic Resource  (4)
  • Carnitine  (2)
  • 127Xe-Radionuclide ventriculography  (1)
  • Adenine  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 100 (1974), S. 219-238 
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nodulation ; Symbiosis ; Adenine ; Thiamine ; Auxotroph ; Prototroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-nodulating auxotroph (L4-73) derived from an effective strain (L4) of Rhizobium leguminosarum has a growth requirement for adenine and thiamine. The auxotroph was able to infect the roots of the host plant Pisum sativum L. but formed root nodules (ineffective in nitrogen fixation) only when adenine and, to a lesser extent, thiamine were added to the plant substrate. Nodules formed in the presence of adenine were structurally abnormal, containing small cells in which infection threads appeared to have aborted. In the presence of thiamine the auxotroph produced a smaller number of nodules which were slightly more developed and were able to reduce trace amounts of acetylene to ethylene. The adenine effect predominated when both growth factors were added together or separately in different sequences. Adenine amendment was required during the first 6 days following inoculation to achieve the maximum number of nodules. The block in symbiosis could not be fully overcome by sequential addition or removal from the root medium of either compound or by addition of some other adeninecontaining compounds. Partial prototroph revertants requiring adenine but not thiamine induced a nodulation response similar to that of the original auxotroph in the presence of thiamine; partial prototrophs requiring thiamine only were almost fully effective. Bioassay of pea root tissue indicated the presence of significant amounts of both adenine and thiamine or related substances in the roots. The auxotroph was able to compete with the parent strain L4 in nodulation on roots of pea only in the presence of exogenous adenine.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 243-248 
    ISSN: 1432-072X
    Keywords: Key words     Lactobacillus plantarum ; Compatible ; solute ; Osmotic stress ; Carnitine ; Betaine ; Acetylcholine ; Succinylcholine ; γ-Butyrobetaine ; Quaternary ammonium compound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The aim of this study was to unravel the identity of compatible solutes accumulated by Lactobacillus plantarum subjected to osmotic stress. Betaine was accumulated simultaneously with a novel compatible solute identified as carnitine, both present in the complex medium applied in this study. Beef extract provided the main source of carnitine in the medium. Both carnitine and betaine were accumulated to maximum concentrations of 248 and 231 μmol.g dry weight–1, respectively. A defined medium was devised devoid of carnitine. Addition of 0.5 mM carnitine to this medium increased the growth rate from 0.1 h–1 to 0.2 h–1 in media with 0.4 M sodium chloride. Also, carnitine made the organism more tolerant to sodium chloride. Growth occurred even when the sodium chloride concentration was raised from 0.5 M to 1.0 M. Quaternary compo unds resembling the structure of carnitine and betaine enhanced the growth yield as well. γ-Butyrobetaine and succinylcholine restored the growth yield up to respectively 91 and 96% compared to non-stressed cells.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 243-248 
    ISSN: 1432-072X
    Keywords: Lactobacillus plantarum ; Compatible solute ; Osmotic stress ; Carnitine ; Betaine ; Acetylcholine ; Succinylcholine ; γ-Butyrobetaine ; Quaternary ammonium compound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this study was to unravel the identity of compatible solutes accumulated by Lactobacillus plantarum subjected to osmotic stress. Betaine was accumulated simulataneously with a novel compatible solute identified as carnitine, both present in the complex medium applied in this study. Beef extract provided the main source of carnitine in the medium. Both carnitine and betaine were accumulated to maximum concentrations of 248 and 231 μmol.g dry weight-1, respectively. A defined medium was devised devoid of carnitine. Addition of 0.5 mM carnitine to this medium increased the growth rate from 0.1 h-1 to 0.2 h-1 in media with 0.4 M sodium chloride. Also, carnitine made the organism more tolerant to sodium chloride. Growth occurred even when the sodium chloride concentration was raised from 0.5 M to 1.0 M. Quaternary compounds resembling the structure of carnitine and betaine enhanced the growth yield as well. γ-Butyrobetaine and succinylcholine restored the growth yield up to respectively 91 and 96% compared to non-stressed cells.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1619-7089
    Keywords: 127Xe-Radionuclide ventriculography ; Right ventricular function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report the development of a new method for obtaining right ventriculograms using the peripheral intravenous infusion of a new tracer, 127Xe dissolved in saline. This tracer has a half life of 36 days, emits 172 keV and 203 keV photons, and is completely cleared by the lungs during pulmonary transit. The right ventricle can therefore be imaged free from interfering activity in the systemic circulation. The technique was used in 11 normal subjects and the results compared with those obtained using first pass and gated equilibrium blood pool angiography with 99mTc. Excellent images of the right ventricle were obtained and the tricuspid and pulmonary valve planes could be easily identified. This imaging technique has significant advantages over existing methods for the noninvasive assessment of right ventricular function.
    Type of Medium: Electronic Resource
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