ISSN:
0749-1581
Keywords:
NMR
;
1H NMR
;
13C NMR
;
insulin dimer
;
secondary structure
;
inverse detection
;
linear prediction extrapolation
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The resonances of the proton-bearing 13C nuclei at natural abundance were assigned for the solution dimer of the B9(Asp) mutant of human insulin. The assignments are based on a combination of direct and relayed 1H—13C correlated spectra, i.e. SQC, SQC-HOHAHA and HMQC-COSY, and the previously assigned 1H spectrum. The study proves valuable not only because of the 13C assignments, but also because it provides a verification, a completion and a few corrections of the previous 1H assignments. Finally, the obtained 13C assignments support the previously reported correlation between the secondary 13Cα and 13Cβ chemical shifts and the secondary structure of proteins.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260330610
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