ISSN:
1432-0983
Keywords:
ARS1
;
2 μm miniplasmids
;
Stability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary With a view to developing a transformation system for brewing yeasts, the stabilities of all-yeast DNA miniplasmids in isogenic haploids were investigated. The stability of the ARS-based miniplasmid YARp11 was found to be comparable to that of two 2 μm ori-based miniplasmids pLAB1 and pLAB2 in selective media, but it propagated at a much higher copy number. The presence or absence of host 2 μm DNA had little effect on the stability of YARp11. The stabilities of all these plasmids were significantly increased in diploid cells. However, a reduction in copy number of the plasmids was observed in all three cases, suggesting influence of MAT genes in controlling plasmid copy number and stability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00420722
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