ISSN:
1573-0972
Keywords:
β-1,4-galactosyltransferase
;
fusion protein
;
glycoprotein processing
;
maltose binding protein
;
man9-mannosidase
;
polymerase chain reaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A truncated Man9-mannosidase gene and a full-length β-1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018570604788
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