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  • Electronic Resource  (2)
  • Abstracts EGF receptor  (1)
  • phosphatidylcholine cycle  (1)
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  • Electronic Resource  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Enhanced expression of EGF receptor and low frequency of ras mutations in X-ray-induced rat thyroid tumours (1999)
    Springer
    Virchows Archiv 435 (1999), S. 434-441 
    Details
    ISSN: 1432-2307
    Keywords: Abstracts EGF receptor ; ras ; Radiation ; Rat thyroid tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Radiation is recognized as a carcinogenic factor for the thyroid gland. In this experimental study, oncogene expression was investigated in radiation-induced rat thyroid tumours. Forty 3-month-old Wistar rats received X-ray-irradiation to the neck region; 40 animals were untreated controls. After 14 months, thyroid tumours had developed in 25 of the 29 irradiated animals still alive; 76% of these tumours were considered malignant. No tumours developed in controls. Mutations of codons 12–13 and 59–63 of H-, K- and N-ras were analysed by PCR-SSCP (single-strand conformation polymorphism analysis) and sequencing of DNA from thyroid tissue. SSCP indicated a ras mutation frequency of 8%, but only one K-ras codon 12 (Gly-Cys) mutation was confirmed by sequencing. Protooncogene expression was analysed by mRNA slot blot hybridization analysis and immunohistochemistry. K-ras mRNA expression and EGF receptor mRNA and protein expression were significantly increased in the irradiated animals compared with controls, and in tumours versus nontumour tissue. This study of radiation-induced rat thyroid tumours demonstrates that ras expression may be subject to changes apart from activating mutations. Increased expression of EGF receptor in the tumours parallels the situation in human thyroid cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008191
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester- treated C3H10T1/2 C18 fibroblasts (1998)
    Springer
    Molecular and cellular biochemistry 187 (1998), S. 147-154 
    Details
    ISSN: 1573-4919
    Keywords: C3H10T1/2 Cl 8 cell line ; phosphatidylcholine cycle ; phospholipase D ; 12-O-tetradecanoylphorbol 13-acetate ; choline ; protein kinase C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have shown that 12-O-tetradecanoylphorbol 13-acetate (TPA) increases protein kinase C (PKC)-mediated choline transport, incorporation of choline into phosphatidylcholine (PtdCho) and PtdCho degradation by phospholipase D (PLD) in C3H10T1/2 Cl 8 cells. Dual prelabeling experiment using [3H]/[14C]choline indicated that intracellular choline generated from the PLD reaction was not directly recycled to PtdCho synthesis within the cell, and that a large fraction of the choline was transported out of the TPA-treated cells. In contrast, medium derived choline was preferably channeled to PtdCho synthesis. These results indicate that in TPA-treated cells, the choline derived from the PKC-mediated increased PLD activity and the choline newly taken up by the cell behave as two distinctly different metabolic pools.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006813524791
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    Paper (German National Licenses)
    Fulltext
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