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Fluorescence label studies of the phase transitions of T7 (1983)

Fidy, J. ; Mauss, Y. ; Pataki, K. ; [et al.]

Springer

European biophysics journal 10 (1983), S. 109-119

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ISSN: 1432-1017

Keywords: Phase transitions of phage T7 ; Light scattering corrected optical, and fluorescence melting ; DPH bound to phage proteins ; Acridine dyes ; Superhelical structure

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Physics

Notes: Abstract Optical density, fluorescence intensity, polarization and lifetime measurements were performed to analyze the temperature-induced phase transitions of phage T7 in several buffers. For labelling the intraphage DNA, ethidium bromide, proflavine and rivanol were applied for the proteins 1,6-diphenyl-1,3,5-hexatriene (DPH). In the temperature range of 20

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00537553

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