ISSN:
1435-2451
Keywords:
Key words Organ preservation
;
Transplantation
;
Na+/K+ ATPase
;
Animal
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Background: Clinically, intracellular type solutions are the most widely used solutions to preserve organs. The optimal ion composition of preservation solutions, however, is still unknown and extracellular-type solutions have frequently been superior to intracellular solutions in various experimental studies. Materials and methods: In this study, we measured extracellular (interstitial) electrolyte concentrations in rat livers, kidneys, hearts and lungs at 4°C by means of microdialysis sampling. Results: After 24 h cold ischaemia, [Na+]int and [K+]int were 104±25 mmol/l and 6.5±0.7 mmol/l in hearts, 92±12 mmol/l and 6.9±1.0 mmol/l in livers, 115±22 mmol/l and 6.3±0.9 mmol/l in kidneys and 87±17 mmol/l and 6.4±0.6 mmol/l in lungs. After preservation of organs in intracellular-type solutions, [Na+]int was significantly lower for each organ (range from 69±8 mmol/l to 73±20 mmol/l) and [K+]int was significantly higher (range from 8.0±1.7 mmol/l to 9.8±1.0 mmol/l). In no instance did the interstitial electrolyte concentration equilibrate with the intracellular electrolyte concentration. When the diffusion gradient from the vascular space to the interstitial space was calculated for Na+ and K+, a significantly higher barrier was found for K+ than for Na+ (P〈0.001 and P〈0.01 for hearts). Conclusions: These studies indicate that during cold storage of rat hearts, lungs, livers and kidneys, intra- and extracellular electrolytes do not equilibrate. Ion exchange stabilises at extracellular Na+ concentrations between 87 mmol/l and 115 mmol/l and K+ concentrations between 6.3 mmol/l and 6.9 mmol/l. Storage of organs in solutions with extracellular-type ion compositions might improve graft function and survival not only after lung and liver but also after heart and renal preservation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004230050267
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