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  • Electronic Resource  (3)
  • Spinach  (2)
  • Anther culture  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Plastocyanin is encoded by an uninterrupted nuclear gene in spinach (1986)
    Springer
    Current genetics 11 (1986), S. 171-176 
    Details
    ISSN: 1432-0983
    Keywords: Plastocyanin ; Photosynthesis ; Genomic and cDNA clones ; Sequence analysis ; Transcript ; Spinach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plastocyanin is a member of photosynthetic electron transport chains that transfers electrons from cytochrome f to the oxidized P700 chlorophyll a pigment of the photosystem I reaction center. We have isolated and characterized cDNA- and genomic clones from spinach (Spinacia oleracea) encoding the complete plastocyanin-precursor polypeptide. The amino acid sequence derived from the nucleotide sequence shows that the precursor consists of 168 amino acid residues including a transit sequence of 69 residues. The precursor polypeptide has a predicted Mr of 16,917, the mature protein of 10,413. The available data indicate that plastocyanin derives probably from a single-copy gene. The coding region contains no intron. The size of the mRNA as determined by S1 nuclease protection experiments is approximately 660 nucleotides, although analysis of different cDNA clones suggests that longer RNA species do exist, approaching the size of the mRNA (850 bases) estimated by Northern blot techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420603
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Induction of haploidy from pollen grains in angiosperms — the current status (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 193-206 
    Details
    ISSN: 1432-2242
    Keywords: Haploids ; Anther culture ; Pollen culture ; Physiological factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Since the successful induction of haploids from anthers cultured in vitro in 1964, a great deal of attention has been given to this problem by those interested in obtaining pure lines and mutants for crop improvement and biochemical genetics. In the last 16 years the anther culture technique has been refined and extended to over one hundred and fifty different species. More recently, isolated pollen culture — which is a refinement of the original anther culture technique — has also been developed. In this review we have made an effort to critically examine existing reports with the objective of analysing the effects of various factors — e.g. culture medium, the cultural conditions, and the effect of genotype and physiological state of the parent plant on pollen induction — and to speculate on the mechanism of action of different factors in order to throw some light on the process of haploid induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288437
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of cDNA clones encoding the complete “33 kDa” precursor protein associated with the photosynthetic oxygen-evolving complex from spinach (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 288-293 
    Details
    ISSN: 1617-4623
    Keywords: Photosynthetic oxygen evolution ; “33 kDa” protein ; cDNA nucleotide sequence ; Transit peptide ; Spinach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several cDNA clones encoding the “33 kDa” protein associated with the photosynthetic water oxidation activity of spinach were sequenced. A 1208 bp insert of one of the clones encodes the entire 331 amino acid residues of the precursor protein including 84 amino acids (8.5 kDa) of the amino-terminal transit peptide, 49 bp of the 5′ and 111 bp of the 3′ untranslated segment of the mRNA. The 3′ poly(A) tail starts 19 bp downstream from a putative polyadenylation signal, TATAAA. The hydrophilic mature protein consists of 247 amino acid residues corresponding to an Mr of 26.5 kDa, which is 6.5 kDa smaller than the value determined by SDS-polyacrylamide gel electrophoresis (33–34 kDa), and shows a certain degree of conservation with the putative Mn-complexing active sites of bacterial Mn-dependent superoxide dismutases. The anatomy of the unusually long transit sequence is discussed with regard to current concepts of protein import into and protein routein within the organelle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331591
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    Paper (German National Licenses)
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