ISSN:
1476-5535
Keywords:
Keywords: hNGF; Luc; PCR; baculovirus system; transfer vector; gene expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Human nerve growth factor (hNGF) gene was proliferated with human leucocyte DNA as template by PCR. Then a fusion gene coding hNGF and luciferase (Luc) cDNAs was inserted into transfer vector pSXIVVI+X3/3 with the control of Syn XIV promoter. Luc and hNGF were simultaneously synthesized in Spodoptera larvae upon infection with a recombinant baculovirus, TnNPV-Luc-NGF-OCC+. Densitometric scanning of SDS-PAGE revealed that ∼18% of the total Coomassie blue-stainable protein of the infected larvae was represented by Luc protein, while the hNGF level was ∼8%. Both proteins were similar to their authentic counterparts in terms of immunoreactivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/sj.jim.2900579
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