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  • Electronic Resource  (5)
  • Brassica  (3)
  • ELISA  (2)
  • detection  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Pollen-pistil interaction in Brassica oleracea (1979)
    Springer
    Planta 146 (1979), S. 211-216 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Pollen adhesion ; Pollen hydration ; Self-incompatibility ; Tryphine mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative studies of the adhesion of pollen grains to the stigma in Brassica oleracea revealed that self-pollen is initially less firmly bound than cross-pollen. The pollen grain tryphine, believed to be important in the adhesion process, has been shown to differ in mobility following self- and cross-pollination when observed using fluorescent probes. The hydration of the pollen grains has been investigated in vitro by measuring the changes in shape, volume and fresh weight of the imbibing grains. Whilst little change in volume could be detected there was a considerable increase in fresh weight together with a change of shape. The significance of these events, which occur prior to pollen germination, is discussed in relation to their effect upon subsequent germination and expression of self-incompatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388234
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A glycoprotein associated with the acquisition of the self-incompatibility system by maturing stigmas of Brassica oleracea (1979)
    Springer
    Planta 146 (1979), S. 179-183 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Cellular recognition ; Glycoproteins ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iso-electric focusing of extracts derived from stigmatic homogenates of Brassica oleracea reveals that the mature stigma possesses large quantities of a glycoprotein not present in earlier stages of development in the bud. Pollen germination experiments carried out in parallel with the biochemical tests suggest that the appearance of this glycoprotein, which has an isoelectric point of pH 5.8, is coincident with the development of the self-incompatibility response. The site of this protein, and the role it may play in pollen-stigma interactions are discussed in terms of current models of the self-incompatibility system in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388229
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Pollen stigma interactions in Brassica oleracea (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 241-246 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Pollen germination ; Pollen surface ; Self-incompatibility ; Stigma surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent studies on the mechanism of self-incompatibility in Brassica indicate the location, nature and mode of action of the molecules involved. Characteristics of the pollen surface and the stigma surface are described in detail, together with new information pertaining to the recognition molecules located therein. A sequence of events is outlined leading from pollination, through adhesion, hydration, germination, and tube growth to acceptance and ultimate compatibility. The characteristics of rejection of incompatible grains are described for each stage of the pollen-stigma interaction. It is proposed that recognition of proteins from the coating of self-pollen by the molecules in the pellicle results in the formation of a biologically-active complex which inhibits water supply to the incompatible grain, and that all other manifestations of incompatibility are a consequence of this initial response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265173
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers grown from seed pieces with various levels of inoculum (1992)
    Springer
    Potato research 35 (1992), S. 207-216 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was used to monitor potato stems of three cultivars for the presence of the ring rot pathogen,Clavibacter michiganensis subsp.sepedonicus, during the growing season and in progeny tubers after harvest. The highest ELISA values were obtained with the highest concentration of bacteria used to inoculate seed pieces in all cultivars tested. Low ELISA values were obtained for stems and progeny tubers selected from plants grown from seed inoculated at lower bacterial concentrations. Estimates of bacterial densities in stems and progeny tubers by immunofluorescence indicated that low ELISA values were most probably caused by low bacterial numbers. It is suggested that the sensitivity of ELISA for detecting the ring rot pathogen in potato stems and progeny tubers is a function of the concentration of bacteria in individual seed pieces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357617
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A comparative study in five laboratories on detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers (1992)
    Springer
    Potato research 35 (1992), S. 217-226 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; eggplant ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato stems and tubers grown in the field from seed tubers inoculated withClavibacter michiganensis subsp.sepedonicus, which causes bacterial ring rot, were tested by indirect, sandwich enzyme-linked immunosorbent assay (ELISA) in five laboratories. Correlation between values for each experimental treatment from the five laboratories was greater (r=0.86) than correlation between values for individual samples (r=0.71). When three or more laboratories obtained ELISA values of ≥0.200 for a sample, that sample was presumed to be positive. Conversely, when three or more laboratories obtained ELISA values 〈0.200, the consensus determination was regarded as negative. The percentage of stem and tuber samples that were in agreement with the consensus ELISA determination varied from 65.5 to 96.7%. Indirect immunofluorescence tests, conducted on the same samples in two laboratories, were consistent with 83.4–91.9% of the consensus ELISA determinations. Presence or absence ofC.m. sepedonicus was confirmed in some samples by an eggplant bioassay and direct isolatiion of the bacterium. The ELISA procedure was well suited for screening large numbers of samples and this study confirms it to be a promising procedure in routine indexing of seed potatoes forC.m. sepedonicus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357618
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    Paper (German National Licenses)
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