ISSN:
1432-2307
Keywords:
Glomerulonephritis
;
Nephritogenic antigen
;
Glomerular basement membrane
;
Type IV collagen
;
NC1
;
Monoclonal antibody
;
Goodpasture's syndrome
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1–α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1–α2(IV)NC1. Renal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1–α6(IV)NC1, and the placental fraction had a large amount of α1–α2(IV)NC1 and a very small amount of α3–α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1–α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular BM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3–α5(IV)NC1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00196702
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