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  • 1
    ISSN: 1432-1017
    Keywords: Cephalopod ; Retina ; Photoreceptor ; Potentials ; Cations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The ERG of the isolated, superfused half-eye of the cephalopod Sepiola atlantica, evoked by a brief (10 Μs) light flash, has been studied by recording intraretinal potentials with glass microelectrodes. The intensity-response characteristics of the potentials recorded at an electrode fixed at the surface (V s ) can be fitted by a simple equation derived from an equivalent circuit model based on a sodium conductance increase mechanism. Raising the external potassium level reduces the maximal response (δV m ), but does not alter the half-saturation intensity value (I 0). Reducing external sodium does not affect (δV m ), but increases I 0. Reducing external calcium also does not affect (δV m ), but decreases I 0. These effects are adequately described by the model if it is also assumed (a) that changing the external sodium does not significantly alter the transmembrane sodium gradient, and (b) that sodium and calcium ions compete for the sensitivity control mechanism. Differential-depth recording between the fixed electrode at the surface and another electrode that could be moved into the retina revealed that the two component appearance of the transretinal ERG arose from the superposition of two vitreal-negative waveforms. An initial “fast” component was mainly recorded in the photoreceptive distal segments while a “slow” component was prominent in the more proximal regions of the retina. Perfusion with high K+ salines resulted in a decrease in the amplitudes of both fast and slow components of the response whereas reducing external Na+ reduced the amplitude of the fast component at all light intensities but reduced the amplitude of the slow component only at low intensities. The amplitudes of both the fast and slow components increased on reducing external calcium, but the rate of rise and fall of the fast component was independent of external calcium. The rate of rise of the slow component was also independent of the external Ca2+ level but a minimum in the recovery time (t F ) was shifted to a lower intensity value at lower calcium concentrations. The shift of the minimum was to a higher intensity value with lowered sodium perfusing solutions. On the basis of the differential sensitivity of the two components to ion changes, as well as stimulus intensity and intraretinal distribution of the components, it is suggested that they reflect two distinct processes in the light-evoked potential of the photoreceptor cells.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: Glomus fasciculatum ; Glomus intraradices ; Glomus macrocarpum ; mineral nutrition ; soil temperature ; Sorghum bicolor ; uptake of P, K, S, Fe, and Zn ; VAMF ; vesicular-arbuscular mycorrhizal fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sorghum [Sorghum bicolor (L.) Moench] plants were grown in growth chambers at 20, 25 and 30°C in a low P Typic Argiudoll (3.65 µg P g−1 soil, pH 8.3) inoculated with Glomus fasciculatum, Glomus intraradices, and Glomus macrocarpum to determine effects of vesicular-arbuscular mycorrhizal fungi (VAMF) species on plant growth and mineral nutrient uptake. Sorghum root colonization by VAMF and plant responses to Glomus species were temperature dependent. G. macrocarpum colonized sorghum roots best and enhanced plant growth and mineral uptake considerably more than the other VAMF species, especially at 30°C. G. fasciculatum enhanced shoot growth at 20 and 25°C, and mineral uptake only at 20°C. G. intraradices depressed shoot growth and mineral uptake at 30°C. G. macrocarpum enhanced shoot P, K, and Zn at all temperatures, and Fe at 25 and 30°C above that which could be accounted for by increased biomass. Sorghum plant growth responses to colonization by VAMF species may need to be evaluated at different temperatures to optimize beneficial effects.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5036
    Keywords: Glomus fasciculatum ; phosphorus nutrition ; Sorghum bicolor (L.) ; Moench ; VA mycorrhizas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sorghum [Sorghum bicolor (L.) Moench] was grown in a greenhouse in a low P (3.6 mg kg-1) soil (Typic Argiudolls) inoculated with the vesicular-arbuscular mycorrhizal fungi (VMAF) Glomus fasciculatum and P added at 0, 12.5, 25.0, and 37.5 mg kg-1 soil to determine the effects of VAMF-root associations on plant growth, benefit and cost analysis, and P efficiency (dry matter produced/unit P absorbed). Root colonization with VAMF and shoot growth enhancements decreased with increased soil P applications. Mycorrhizal plants were less P efficient than nonmycorrhizal plants. Shoot dry matter differences between mycorrhizal and nonmycorrhizal plants were considered the benefit derived by plants from VAMF-root associations. Shoot dry matter differences between mycorrhizal and nonmycorrhizal plants with similar P concentrations were considered the costs paid by plants for VAMF-root associations. Values of benefit and cost analysis for VAMF-root associations were highest when soil P was lowest and decreased with increasing P applications. Genotypic differences for calculated costs were pronounced, but not benefits. Benefit and cost analysis.may be helpful to evaluate host plant genotypes and VAMF species to optimize efficiencies of VAMF symbiosis in different soil environments.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: Glomus fasciculatum ; Glomus intraradices ; Glomus macrocarpum ; mineral nutrition ; soil temperature ; Sorghum bicolor ; uptake of P, K, S, Fe, and Zn ; VAMF ; vesicular-arbuscular mycorrhizal fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sorghum [Sorghum bicolor (L.) Moench] plants were grown in growth chambers at 20, 25 and 30°C in a low P Typic Argiudoll (3.65 µg P g-1 soil, pH 8.3) inoculated withGlomus fasciculatum, Glomus intraradices, andGlomus macrocarpum to determine effects of vesicular-arbuscular mycorrhizal fungi (VAMF) species on plant growth and mineral nutrient uptake. Sorghum root colonization by VAMF and plant responses toGlomus species were temperature dependent.G. macrocarpum colonized sorghum roots best and enhanced plant growth and mineral uptake considerably more than the other VAMF species, especially at 30°C.G. fasciculatum enhanced shoot growth at 20 and 25°C, and mineral uptake only at 20°C.G. intraradices depressed shoot growth and mineral uptake at 30°C.G. macrocarpum enhanced shoot P, K, and Zn at all temperatures, and Fe at 25 and 30°C above that which could be accounted for by increased biomass. Sorghum plant growth responses to colonization by VAMF species may need to be evaluated at different temperatures to optimize beneficial effects.
    Type of Medium: Electronic Resource
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