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  • Electronic Resource  (5)
  • Cell & Developmental Biology  (3)
  • ELISA  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers grown from seed pieces with various levels of inoculum (1992)
    Springer
    Potato research 35 (1992), S. 207-216 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was used to monitor potato stems of three cultivars for the presence of the ring rot pathogen,Clavibacter michiganensis subsp.sepedonicus, during the growing season and in progeny tubers after harvest. The highest ELISA values were obtained with the highest concentration of bacteria used to inoculate seed pieces in all cultivars tested. Low ELISA values were obtained for stems and progeny tubers selected from plants grown from seed inoculated at lower bacterial concentrations. Estimates of bacterial densities in stems and progeny tubers by immunofluorescence indicated that low ELISA values were most probably caused by low bacterial numbers. It is suggested that the sensitivity of ELISA for detecting the ring rot pathogen in potato stems and progeny tubers is a function of the concentration of bacteria in individual seed pieces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357617
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  • 2
    Electronic Resource
    Electronic Resource
    A comparative study in five laboratories on detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers (1992)
    Springer
    Potato research 35 (1992), S. 217-226 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; eggplant ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato stems and tubers grown in the field from seed tubers inoculated withClavibacter michiganensis subsp.sepedonicus, which causes bacterial ring rot, were tested by indirect, sandwich enzyme-linked immunosorbent assay (ELISA) in five laboratories. Correlation between values for each experimental treatment from the five laboratories was greater (r=0.86) than correlation between values for individual samples (r=0.71). When three or more laboratories obtained ELISA values of ≥0.200 for a sample, that sample was presumed to be positive. Conversely, when three or more laboratories obtained ELISA values 〈0.200, the consensus determination was regarded as negative. The percentage of stem and tuber samples that were in agreement with the consensus ELISA determination varied from 65.5 to 96.7%. Indirect immunofluorescence tests, conducted on the same samples in two laboratories, were consistent with 83.4–91.9% of the consensus ELISA determinations. Presence or absence ofC.m. sepedonicus was confirmed in some samples by an eggplant bioassay and direct isolatiion of the bacterium. The ELISA procedure was well suited for screening large numbers of samples and this study confirms it to be a promising procedure in routine indexing of seed potatoes forC.m. sepedonicus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357618
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The histogenesis of muscle in the regenerating fore limb of larval Amblystoma punctatumThesis presented to the faculty of Princeton University in partial fulfillment of the requirements for the degree of doctor of philosophy. (1938)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 62 (1938), S. 17-47 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The regeneration of muscle in larval Amblystoma punctatum is preceded by an extensive dedifferentiation of the old muscles of the limb stump. The process of muscle dedifferentiation consists in a separation of muscle nuclei, surrounded by a small amount of cytoplasm, from the injured ends of the muscle fibers. The dedifferentiation of the cut muscles of the limb stump progresses proximad as far as the origin of the muscles on the humerus and results in a complete transformation of these muscles into undifferentiated cells which appear to contribute to the formation of the regeneration blastema. Shoulder muscles, which were attached to the humerus, also undergo a partial dedifferentiation when their points of insertion on the humerus are destroyed by the degeneration of the perichondrium. These muscles never dedifferentiate, however, for more than one-fourth their original length. The process of dedifferentiation in the shoulder muscles is similar to that found in the cut muscles of the limb stump.The regeneration of the injured muscles occurs in two ways. The shoulder muscles reconstitute themselves by means of terminal and lateral sarcoplasmic buds formed near the distal regions of the muscle fibers. The muscles of the limb proper, distal to the shoulder, differentiate out of local aggregations of blastema cells. No myoblasts were observed.The regeneration blastema arose chiefly from dedifferentiated cells of muscle, nerve connective tissue sheath, perichondrium and cartilage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050620104
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  • 4
    Electronic Resource
    Electronic Resource
    Beryllium inhibition of regeneration. I. Morphological effects of beryllium on amputated fore limbs of larval Amblystoma (1949)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 84 (1949), S. 459-493 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050840305
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  • 5
    Electronic Resource
    Electronic Resource
    The histogenesis of the regenerating fore limb of larval Amblystoma after exarticulation of the humerusThesis presented to the faculty of Princeton University in partial fulfillment of the requirements for the degree of Doctor of Philosophy. (1938)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 62 (1938), S. 219-241 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A new limb skeleton will form in regenerating limbs of the larval Amblystoma in which the humerus had been completely removed. The cartilage of the new limb skeleton develops out of a blastema in which there are no cells of cartilage origin. This regeneration blastema is a composite structure made up of cells derived from a dedifferentiation of the injured tissues of the limb. The tissues which have been observed as contributing to the regeneration blastema are: muscles, especially the muscles of the shoulder; connective tissue of the sheath of the brachial nerve plexus; muscle connective tissue; and, to a certain extent, subcutaneous connective tissue. The new cartilage of the limb skeleton develops out of this composite blastema by means of a differentiation of cells in the central axis of the blastema.The amount of cartilage regenerated appears to depend on the mass of the blastema. When the blastema does not extend fully into the glenoid cavity of the scapula, the head of the developing humerus is deficient in size and structure. However, a complete limb skeleton is regenerated when the blastema does extend fully into the glenoid cavity.Since the new cartilage of the regenerated limb has no genetic continuity with the old limb skeleton, it would appear that the limb field exerts some kind of histogenetic determining action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050620204
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    Paper (German National Licenses)
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