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  • Electronic Resource  (2)
  • Cell division cycle  (1)
  • Chilomastix cuspidata  (1)
  • 1
    ISSN: 1615-6102
    Keywords: Centrin ; Chilomastix cuspidata ; Cytoskeleton ; Protist ; Tubulin ; Retortamonad
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immunochemical techniques were used to identify and localise centrin and α-tubulin inChilomastix cuspidata, an amitochondriate “excavate flagellate”. Immunoblotting showed that the molecular mass of centrin is 20 kDa and α-tubulin is 55 kDa. Immunofluorescence microscopy localises centrin to the basal-body region of the flagellar apparatus and in a centrin-containing root which runs along the groove towards the posterior of the cell. Confocal microscopy of cells double-labelled with anticentrin and antitubulin antibodies suggests that centrin is also associated with a group of microtubules called the hook band. Centrin labelling extends for two thirds of the length of the hook band, ending in a pill-like structure. In all we distinguish five major components of the centrin root. The distribution of these proteins is discussed in terms of the functions centrin and tubulin may play in this putatively primitive protist and its relatives.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Basal-body root complex ; Cell division cycle ; Centrosome ; Chlamydomonas ; Evolution ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mammalian centrosome monoclonal antibody MPM-13 recognized component(s) of the well defined MTOC basal-body root complex in the green plantChlamydomonas. The antibody reaction coincided in location with the basal-body root complex and the cruciate nature of the staining pattern corresponded to the configuration of the root microtubules. During mitosis the behaviour of MPM-13 stained material mirrored the duplication, separation and migration to the spindle poles of the basal body-root complex. It is suggested that conserved MTOC components were recognized and that these may have retained a similar, perhaps universal, function in microtubule organization.
    Type of Medium: Electronic Resource
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