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  • Electronic Resource  (16)
  • Chemistry  (12)
  • Interspecific hybridization  (2)
  • Key words Triticum aestivum  (2)
  • 1
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Blumeria graminis f. sp. tritici ; QTL mapping ; Molecular markers ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Powdery mildew (Blumeria graminis f. sp. tritici) is one of the most damaging diseases of wheat (Triticum aestivum). The objective of this study was to locate and map a recently identified powdery mildew resistance gene, MlRE, carried by the resistant line RE714 using microsatellites uniformly distributed among the whole genome together with a bulked segregant analysis (BSA). The bulks consisted of individuals with an extreme phenotype taken from a population of 140 F3 families issued from the cross between RE714 (resistant) and Hardi (susceptible). The population had been tested with three powdery mildew isolates at the seedling stage. Qualitative interpretation of the resistance tests located the MlRE gene on the distal part of the long arm of chromosome 6A. A subsequent quantitative interpretation of the resistance permitted us to detect another resistance factor on a linkage group assigned to chromosome 5D, which was constructed with microsatellites for which a polymorphism of intensity between bulks was observed. This quantitative trait locus (QTL) explained 16.8– 25.34% of the total variation. An interaction between both the resistant factor (MlRE and the QTL) was found for only one of the isolates tested. This study shows the advantage of making a quantitative interpretation of resistant tests and that the use of microsatellites combined with BSA is a powerful strategy to locate resistance genes in wheat.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Tetraploid triticale ; Chromosome pairing ; Interspecific hybridization ; Genome re-arrangement ; Genome affinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two F5 strains of tetraploid triticale (2n= 4x=28), obtained from 6x triticaleX2 rye progenies, were crossed with diploid and tetraploid rye, some durum and bread wheats, and various 8x and 6x triticale lines. Meiosis in the different hybrid combinations was studied. The results showed that the haploid complement of these triticales consists of seven chromosomes from rye and seven chromosomes from wheat. High frequencies of PMCs showing trivalents were observed in hybrids involving the reference genotypes of wheat and triticale. These findings proved that several chromosomes from the wheat component have chromosome segments coming from two parental wheat chromosomes. The origin of these heterogeneous chromosomes probably lies in homoeologous pairing occurring at meiosis in the 6x triticaleX2x rye hybrids from which 4x triticale lines were isolated. A comparison among different hybrids combinations indicated that the involvement of D-genome chromosomes in homoeologous pairing is quite limited. In contrast, meiotic patterns in 4x triticale X 2x rye hybrids showed a quite high pairing frequency between some R chromosomes and their A and B homoeologues.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 646-653 
    ISSN: 1432-2242
    Keywords: Triticale ; Interspecific hybridization ; Genome rearrangement ; Biochemical markers ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifty-two progenies originating from a cross between 8x and 4x triticale were submitted to cytogenetic analyses and to various electrophoretic studies [high-molecular-weight (HMW) glutelins, HMW secalins, γ-secalins, α and ω-gliadins, β-amylases] to identify new genetic structures, more specifically the input of the D genome in a genetic context other than the wheat one. Markers of the rye genome (HMW and ω-secalins) were identified in all of the triticale lines, but they originated either from the 4x or from the 8x parent, or from both. Chromosomes 4A, 1B, and 2R, present in both parents, showed the same banding patterns in all progenies. Chromosomes 1R and 5R, present in both parents, showed heterogeneous labelling. The expression of chromosomes 6A, 1D, and 4D, present in the 8x parent only, was more complex with a possible involvement of a regulatory system. Several hexaploid progenies had introgressed part of the D genome, suggesting that crossing 8x and 4x triticale was a practicable approach for transferring D chromosomes into hexaploid triticale.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Doubled haploids   ; Intervarietal map ; Distortion segregation ; Genetic map comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An intervarietal molecular marker map covering most of the nuclear genome was developed in Triticum aestivum. One hundred and six androgenetic-derived doubled haploid lines obtained from the F1 between monosomics of ‘Chinese Spring’ and ‘Courtot’ were analysed for genetic mapping. The map covered 18 of the 21 chromosomes with an identical distribution of markers in the A and B genome, and only small segments of the D genome. Distorted markers were mapped using Bailey’s 2-point method and revealed skewed regions on 1A, 1DS, 2A, 2B, 4AS and 6B. Comparison with a wide cross [‘Opata’×Synthetic hexaploid (T. tauschii/‘Altar 84’)] showed colinearity for markers on homologous chromosomes, but revealed a large proportion (25%) of markers mapped on non-homoeologous chromosomes, i. e. heterologous markers. The origin of the material and distortion segregation are discussed with particular emphasis on investigations of D-genome markers.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Biopolymers 25 (1986), S. 1087-1093 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Solvent accessible peptide bonds in proteins exhibit a 1-3° compression of the OCN bond angle and a corresponding expansion of the NCCa bond angle, relative to buried peptide bonds. These changes are consistent with an increase in hydrogen bonding to the carbonyl oxygen accompanying solvent exposure (J. D. Dunitz and F. K. Winkler, (1975) Acta Cryst. B31, 251-263). For amphiphilic structures such as α-helices, systematic differences in peptide-bond geometry between solvent-exposed and buried residues will generate significant curvature. A decrease of 4° in the OCN bond angle between hydrophilic and hydrophobic sides of an amphiphilic helix will lead to smooth bending, with a radius of curvature of about 70 Å. This curvature is in the range observed for α-helices in proteins. Helix curvature is estimated to have only a small effect on the magnitude and direction of the helical dipole moment.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 1α, 25, 26-trihydroxy metabolite of vitamin D3, isolated from bovine serum, was shown to possess the (25 S)-configuration by HPLC. comparison of the 1, 3, 26-triacetate derivative with authentic (25 R)- and (25 S)-samples. The convergent synthesis of (25 R)-1α, 25, 26- and (25 S)-1α, 25, 26-trihydroxycholecalciferols (10a) and (10b) has been accomplished.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 14 (1968), S. 483-490 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Local and average heat transfer coefficients have been measured for a sphere in a randomly packed bed. A steady state technique was employed in which internally heated spheres were placed in a bed 35 in. deep and 12 in. sq. Air passed through the bed in downflow, the range of Reynolds numbers being from 120 to 1,700 based on the sphere diameter and the superficial velocity.Average heat transfer coefficients have been measured at twenty-five positions permitting the assessment of the effect of position. An entrance region limited to the first two particle layers in the bed has been verified.Distributions of the local heat transfer coefficient on the surface of a single sphere in the top layer and in the nineteenth layer of the bed have also been measured. These distributions indicate that a laminar boundary layer exists over portions of the sphere surface.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 5 (1991), S. 299-302 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The molecular weights of the recombinant proteins interleukin 2 and interferon gamma were determined by electrospray ionization (ESI) mass spectroscopy on the whole molecules. For interleukin 2 an average experimental mass of 15 549.4 u, was observed in excellent agreement with the theoretical average mass of 15 547.2 u. The mass observed for interferon gamma (16 908.4 u) agreed with the theoretical molecular weight of the monomeric species (16 907.3 u). By ESI mass spectrometry it was possible to determine the presence of proteins lacking one or two amino acids.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 331 (1989), S. 600-610 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Azoles. 22. 1H- and 13C-N.M.R. Investigation of Isomeric-3 and 5-Amino-Pyrazole DerivativesThe 1H- and 13C-n.m.r. spectra of nine pairs of isomeric 3- and 5-amino-pyrazole derivatives 1a - 1i and 2a - 2i and some of their acetylderivatives have been assigned and discussed. It has been found that 1H- and 13C-n.m.r. spectroscopy is a useful tool for differentation of the above mentioned 3- and 5-amino-isomers.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Azoles. Mass Spectrometric Studies on Derivatives of β-(Pyrazole-4-yl) Acrylic or -Propionic Acid and Pyrazole-4-yl Methylenemalonic Acid
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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