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  • Electronic Resource  (47)
  • Chemistry  (45)
  • Polymer and Materials Science  (6)
  • Recombinant proteins  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the intrathecal immune response in neuroborreliosis to a sonicate antigen and three recombinant antigens ofBorrelia burgdorferi sensu stricto (1998)
    Springer
    European journal of clinical microbiology & infectious diseases 17 (1998), S. 159-166 
    Details
    ISSN: 1435-4373
    Keywords: Borrelia burgdorferi ; Recombinant proteins ; Enzyme immunoassay ; Antibody index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The intrathecal synthesis of borrelial-specific IgM- and IgG-antibodies was studied in 67 patients with neuroborreliosis and in 14 patients with neurosyphilis (controls). Antibody concentrations in serum and in the cerebrospinal fluid were determined by an enzyme immunoassay (EIA) using, as antigens, a sonicate ofBorrelia burgdorferi, the recombinant 14 kDa flagellin fragment, the outer surface protein C (22 kDa), and the high molecular mass protein p83 (83 kDa). In the sonicate EIA, IgG- and/or IgM-antibodies toBorrelia burgdorferi in serum were detected in all patients with neuroborreliosis and in 71% of patients with neurosyphilis. Intrathecal synthesis of borrelial-specific IgG- and/or IgM-antibodies was demonstrated in 82% of patients with neuroborreliosis and in 71% of patients with neurosyphilis. Immunoglobulin G- and/or IgM-antibodies in serum against any of the recombinant antigens were detected in 92% of patients with neuroborreliosis and in none of those with neurosyphilis. Intrathecal synthesis of IgG- and/or IgM-antibodies to individual recombinant antigens was demonstrated in 67% of patients with neuroborreliosis and in none of those with neurosyphilis. The sensitivity of the recombinant antigens in serum was almost equal to that of the sonicate EIA, whereas the recombinant antigens were clearly less sensitive in the estimation of the intrathecal specific immune response. It was concluded that in suspected cases of neuroborreliosis, the estimation of high specific antibodies in the recombinant EIA will be helpful in confirming the diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01691111
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the Intrathecal Immune Response in Neuroborreliosis to a Sonicate Antigen and Three Recombinant Antigens of Borrelia burgdorferi Sensu Stricto (1998)
    Springer
    European journal of clinical microbiology & infectious diseases 17 (1998), S. 159-166 
    Details
    ISSN: 1435-4373
    Keywords: Key words Borrelia burgdorferi ; Recombinant proteins ; Enzyme immunoassay ; Antibody index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The intrathecal synthesis of borrelial-specific IgM- and IgG-antibodies was studied in 67 patients with neuroborreliosis and in 14 patients with neurosyphilis (controls). Antibody concentrations in serum and in the cerebrospinal fluid were determined by an enzyme immunoassay (EIA) using, as antigens, a sonicate of Borrelia burgdorferi, the recombinant 14 kDa flagellin fragment, the outer surface protein C (22 kDa), and the high molecular mass protein p83 (83 kDa). In the sonicate EIA, IgG- and/or IgM-antibodies to Borrelia burgdorferi in serum were detected in all patients with neuroborreliosis and in 71% of patients with neurosyphilis. Intrathecal synthesis of borrelial-specific IgG- and/or IgM-antibodies was demonstrated in 82% of patients with neuroborreliosis and in 71% of patients with neurosyphilis. Immunoglobulin G- and/or IgM-antibodies in serum against any of the recombinant antigens were detected in 92% of patients with neuroborreliosis and in none of those with neurosyphilis. Intrathecal synthesis of IgG- and/or IgM-antibodies to individual recombinant antigens was demonstrated in 67% of patients with neuroborreliosis and in none of those with neurosyphilis. The sensitivity of the recombinant antigens in serum was almost equal to that of the sonicate EIA, whereas the recombinant antigens were clearly less sensitive in the estimation of the intrathecal specific immune response. It was concluded that in suspected cases of neuroborreliosis, the estimation of high specific antibodies in the recombinant EIA will be helpful in confirming the diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01691111
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  • 3
    Electronic Resource
    Electronic Resource
    Transverse NMR relaxation of water in wood (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 23 (1979), S. 3147-3154 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The transverse NMR relaxation times of hydrogen nuclei of water absorbed in white spruce sapwood [Picea glauca (Meunch) Voss] were measured for moisture contents in the range from 5 to 176%. The spin echo amplitudes resulting from the Carr-Purcell sequence decay nonexponentially suggesting the possibility of at least two different relaxation times for water in wood. A simplified structural model of the wood-water mixture is used to estimate the rates of chemical exchange at room temperature of hydrogen nuclei between various sites in the system. The high-resolution NMR line shape is discussed briefly in terms of this proposed model.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1979.070231101
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  • 4
    Electronic Resource
    Electronic Resource
    Nomenclature discussion (1) (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 113-114 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010216
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  • 5
    Electronic Resource
    Electronic Resource
    Accurate determination of gas holdup time (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 115-120 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010217
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  • 6
    Electronic Resource
    Electronic Resource
    Working range of packed columns and capillary columns in gas chromatography (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 321-326 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010617
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  • 7
    Electronic Resource
    Electronic Resource
    Anticircular high performance thin-layer chromatography (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 164-168 
    Details
    ISSN: 0935-6304
    Keywords: Thin Layer Chromatography, HPTLC ; Fastest possible analytical mode: Anticircular separation ; First principal analytical details ; Most economical TLC/HPTLC technique, quantitative comparison with linear and circular mode ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The principal analytical details for the third of three possible modes in high performance thin-layer chromatography are given, namely the anticircular mode. Separation is achieved by allowing the mobile phase to enter the plate layer on a precise outer circle line, from where it flows towards the centre with nearly constant speed. This technique is theoretically and practically the fastest of all three possible in HPTLC. It permits maximum sample capacity with a minimum of time, layer and mobile phase consumption. It is therefore the most economical HPTLC technique.A new carrier-free mobile phase transfer principle is used. The conditions for qualitative and quantitative analysis are good: repeatability, reproducibility and accuracy of routine TLC analyses are superior to those achieved by the classical trough technique. The specially narrow spot-path in anticircular HPTLC facilitates automated quantitation. Compared with the linear and circular modes, the anticircular mode shows better separation and significantly increased sensitivity at higher Rf-values. The drawback, however, is that the separation power (expressed by the separation number) is lower compared with the other two modes.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010309
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  • 8
    Electronic Resource
    Electronic Resource
    Change of polarity by temperature variation of series-coupled micropacked GC columns, measured in retention index data (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 201-202 
    Details
    ISSN: 0935-6304
    Keywords: Gas Chromatography ; Micropacked columns ; Series-coupled ; Polarity change by temperature variation ; Temperatures independently varied in each column ; Retention index data for study of microprocessor-controlled resolution optimization ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010406
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  • 9
    Electronic Resource
    Electronic Resource
    Polarity change in capillary GC by serial-column temperature optimization (SECAT mode in capillary GC) (1979)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 416-422 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; SECAT-technique=stepless fine tuning of capillary selectivity by serial column temperature optimization ; Exponential influence of temperature on selectivity over a wide range found ; SECAT-technique ready for automation of column adjustment on sample composition polarity ; Instrumental and methodological details for primitive self made solution given ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: GC, including capillary GC, is rather inflexible, if a certain column length and stationary phase has been fixed for a given analytical problem. If the sample composition changes, one often has to change the column length and/or stationary phase, at least when something like optimum analytical conditions are needed. Temperature changes (or heating rates) can change the selectivity of a given column only within very limited ranges, due to the exponential effect of temperature on retention time. By serial coupling two chemically different capillaries, each run at another temperature, even the slightest changes of these two temperature values have a dramatic effect on the selectivity of the system for polar substances. We call this technique the SECAT mode of GC. Results are reported as retention index shifts, dependent on SECAT temperature data. This technique can in future easily be automated, thus enabling the analyst, for polar sample analysis, to adjust a given chroma-tographic system to his specific sample composition without touching the instrument.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240020709
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  • 10
    Electronic Resource
    Electronic Resource
    A simple high temperature, high pressure safe T-piece for installing micro packed glass columns as well as macrobore capillaries in a GC instrument (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 595-595 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Connection device ; Micro packed columns ; Macrobore capillaries ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240091012
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