ISSN:
0377-0486
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
Raman spectra of yeast alcohol dehydrogenase in buffered, aqueous solutions and in D2O, and FTIR spectra of aqueous solutions and thin films have been collected and analyzed. No significant differences between the Raman spectra in the pH range 6.00-7.50 and between the infrared spectra of the solution and film have been observed. The tyrosine doublet strongly favors exposed hydroxyl groups and the Raman spectra indicate multiple conformations for the disulfide moieties. Analysis of Raman intensities favors significant α-helix and random coil contents. The secondary structure of yeast alcohol dehydrogenase based on Raman data is compared with the better characterized secondary structure of equine liver alcohol dehydrogenase.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jrs.1250180210
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