ISSN:
1420-908X
Keywords:
Pleurisy
;
Burn
;
Polymorphonuclear
;
Copper niflumate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Antiinflammatory activities and modulations of PMNL responses produced by treatment with tetrakis-μ-2-[3-(trifluoromethyl)-phenyl] aminonicotinatodicopper (II) [Cu(II)2(niflumate)4] and niflumic acid were studied in isologous serum-induced rat pleurisy. Doses of 10 or 30 mg/kg (35 or 106 µmol/kg) of niflumic acid or Cu(II)2 (niflumate)4 (8 or 23 µmol/kg) caused significant (p 〈 0.01) reductions in pleural exudate and number of polymorphonuclear leukocytes (PMNLs) in the exudate. While both doses of Cu(II)2(niflumate)4 produced significant dose-related reductions in both parameters, only the higher dose of niflumic acid produced a significant dose-related reduction in both parameters. Boyden chamber measurements of N-formyl-methionyl-leucyl-phenylalanine (f-MLP) chemotaxis by PMNLs incubated with 10 or 30 µg/ml niflumic acid (35 or 106 nmol/ml) or Cu(II)2(niflumate)4 (8 or 23 nmol/ml) were significantly (p 〈 0.01 to p 〈 0.001) decreased in dose-related fashions. Chemotaxis of PMNLs from pleuritic rats treated orally with 10 or 30 mg/kg niflumic acid or Cu(II)2(niflumate)4 was significantly (p 〈 0.001) inhibited by the larger dose of niflumic acid and both doses of Cu(II)2(niflumate)4. Opsonized zymosan (OZ)-stimulated chemiluminescence (CL) of PMNLs from pleuritic rats treated orally with these same doses of niflumic acid or Cu(II)2(niflumate)4 was only significantly (p 〈 0.05 or p 〈 0.01 respectively) decreased by the larger doses. Superoxide (O 2 - ) production by these cells was significantly decreased by the larger dose of niflumic acid (p 〈 0.05) while both doses of Cu(II)2(niflumate)4 produced significant (p 〈 0.05 to p 〈 0.01) decreases. Recovery of the decreased PMNL response in burned rats was also studied following treatment with these two compounds. Oral treatment of non-burned rats with 1 mg/kg niflumic acid (4 µmol/kg) or Cu(II)2(niflumate)4 (1 µmol/kg) did not affect OZ-stimulated O 2 - production while decreased O 2 - production in non-treated scald-burned rats was reversed by oral treatment with either niflumic acid or Cu(II)2(niflumate)4. It is concluded that Cu(II)2(niflumate)4 is a more effective antiinflammatory agent than niflumic acid and more effective modulation of PMNL responsiveness may explain its beneficial antipleuritic and burn-injury recovery effects. Formation of the copper complex of niflumic acidin vivo may also account for its beneficial antiinflammatory effects and recovery of depressed PMNL responsiveness in burned rats.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01782258
Permalink
