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Interspecific hybridization of Allium giganteum Regel: production and early verification of putative hybrids (1998)

Dubouzet, J. G. ; Shinoda, K. ; Murata, N.

Springer

Theoretical and applied genetics 96 (1998), S. 385-388

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Details

ISSN: 1432-2242

Schlagwort(e): Key words Alluium giganteum ; Interspecific hybridization ; Cut Flower culture ; Embryo rescue ; Internal transcribed spacer ; Restriction analysis

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Cut flowers of Allium giganteum Regel were emasculated and maintained in half-strength Murashige and Skoog liquid medium supplemented with 3% sucrose and 1000 ppm each of AgrimycinR and BenlateR. Wide hybridization was attempted and, through embryo rescue, putative hybrids were obtained from crosses involving A. cernuum Roth, A. oreophilum C.A. Mey. and A. schubertii Zucc. PCR amplification of the internal transcribed spacer of ribosomal DNA followed by digestion with NdeII generated restriction profiles that confirmed the hybrid nature of the A. giganteum×A. schubertii progenies. The other putative hybrids were found to be products of self pollination.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s001220050753

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