ISSN:
1432-072X
Keywords:
Cytochrome c
;
Cytochrome c peroxidase
;
Nitrite reductase
;
Denitrification
;
Paracoccus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Exposure of aerobically-grown wild-type cells of Paracoccus denitrificans to a decreased aeration caused parallel increases in both PMS/ascorbate and succinate-linked activities of nitrite reductase. By contrast, the expression of the succinate-linked activity was considerably delayed in an insertion mutant specifically lacking the periplasmic 15 kDa cytochrome c-550. In this case the observed activity followed very closely the content of a 40 kDa cytochrome c. A subcellular fraction enriched in a haemoprotein of a similar apparent molecular weight showed the activity of cytochrome c peroxidase and was able to restore the antimycin-sensitive electron transport from membrane vesicles to nitrite reductase. It is concluded that P. denitrificans possesses an alternative nitrite-reducing pathway involving the 40 kDa cytochrome c instead of cytochrome c-550. This pathway branches from the respiratory chain after the cytochrome bc 1 segment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248483
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