Digitale Medien
Springer
Journal of clinical immunology
9 (1989), S. 437-447
ISSN:
1573-2592
Schlagwort(e):
Polymerase chain reaction
;
DNA
;
denaturing gradient gel electrophoresis
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by inserting it into a vector and introducing the vector into a host cell. PCR represents a form of “in vitro cloning” that can generate, as well as modify, DNA fragments of defined length and sequence in a simple automated reaction. In addition to its many applications in basic molecular biological research, PCR promises to play a critical role in the identification of medically important sequences as well as an important diagnostic one in their detection.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00918012
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