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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of thepara-type sodium channel gene from susceptible andkdr-resistant German cockroaches (Blattella germanica) and house fly (Musca domestica) (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 61-68 
    Details
    ISSN: 1617-4623
    Keywords: Blattella germanica ; Para sodium channel gene ; Pyrethroids ; Knockdown resistance (kdr)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using reverse transcription polymerase chain reactions (RT-PCR), the DNA sequence for the main membrane-spanning region (IS3 through IVS6) of the gene encoding theα-subunit of thepara sodium channel of the German cockroach,Blattella germanica, has been determined. The overall structure of the open reading frame region of thisB. germanica gene is very similar to that of thepara gene ofDrosophila melanogaster, and that of the partially sequencedpara gene ofMusca domestica. On the other hand, it is distinctly different from that of theDSC gene (Drosophila sodium channel). As a result of a side-by-side comparison of thepara gene sequences of the susceptible CSMA strain and thekdr resistant VT strain ofB. germanica, one mutation (TTG to TTC) at the approximate center of the IIS6 membrane-spanning segment was found to result in an amino acid change from L to F. While the functional meaning of this mutation for the operation of thepara sodium channel remains to be studied, this region is very highly conserved among all sodium channels identified so far, and is one of the most hydrophobic areas of the entireα-subunit. For comparison, we have studied the same region of thepara sodium channel of bothkdr and susceptible SBO strain of the housefly,Musca domestica. We found the homologous type of mutation, CTT to TTT, resulting in the same amino acid alteration (L to F) at this site. However, in the case of houseflies bothkdr and susceptible strains contained both L and F versions of the protein. The ratio of TTT to CTT was significantly higher in thekdr strain ofM. domestica than in the three susceptible strains examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173205
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of the para-type sodium channel gene from susceptible and kdr-resistant German cockroaches (Blattella germanica) and house fly (Musca domestica) (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 61-68 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsBlattella germanica ; Para sodium channel gene ; Pyrethroids ; Knockdown resistance (kdr)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using reverse transcription polymerase chain reactions (RT-PCR), the DNA sequence for the main membrane-spanning region (IS3 through IVS6) of the gene encoding the α-subunit of the para sodium channel of the German cockroach, Blattella germanica, has been determined. The overall structure of the open reading frame region of this B. germanica gene is very similar to that of the para gene of Drosophila melanogaster, and that of the partially sequenced para gene of Musca domestica. On the other hand, it is distinctly different from that of the DSC gene (Drosophila sodium channel). As a result of a side-by-side comparison of the para gene sequences of the susceptible CSMA strain and the kdr resistant VT strain of B. germanica, on mutation (TTG to TTC) at the approximate center of the IIS6 membrane-spanning segment was found to result in an amino acid change from L to F. While the functional meaning of this mutation for the operation of the para sodium channel remains to be studied, this region is very highly conserved among all sodium channels identified so far, and is one of the most hydrophobic areas of the entire α-subunit. For comparison, we have studied the same region of the para sodium channel of both kdr and susceptible SBO strain of the housefly, Musca domestica. We found the homologous type of mutation, CTT to TTT, resulting in the same amino acid alteration (L to F) at this site. However, in the case of houseflies both kdr and susceptible strains contained both L and F versions of the protein. The ratio of TTT to CTT was significantly higher in the kdr strain of M. domestica than in the three susceptible strains examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004389670007
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Field response and gas-liquid chromatograph separation of optically active synthetic and natural pheromones in two sympatric diprionid sawflies,Neodiprion nanulus nanulus andNeodiprion sertifer (Hymenoptera: Diprionidae) (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 1395-1408 
    Details
    ISSN: 1573-1561
    Keywords: Sawflies ; Neodiprion nanulus ; Neodiprion sertifier ; Hymenoptera ; Diprionidae ; pheromones ; chiral ; chirality ; 3,7-dimethylpentadecan-2-yl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The male red pine sawflyNeodiprion nanulus nanulus Schedl responds mainly to the (2S,3S,7S)-3,7-dimethylpentadecan-2-yl acetate (2S,3S,7S-A) in the field. No other isomer is as effective by itself or syner-gizes 2S,3S, 7S-A in this species. ForNeodiprion sertifer (Geoffroy), however, we confirmed our earlier report that males responded significantly to a 5∶0.003 mixture of 2S,3S,7S-A and either 2S,3R,7R-A or 2S,3R,7R/S-A. The 2S,3S,7S-A isomer was separated from 2S,3R,7R-A by capillary gas-liquid chromatographic (GLC) analysis using Carbowax 20 M and DB-5 columns. The latter column also separated 2S,3S,7S-A from 2S,3R,7S-A. Only 2S,3S,7S-A was found in the natural pheromones ofN. n. nanulus andN. sertifer by GLC analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012286
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Pheromone-dependent species recognition mechanisms betweenNeodiprion pinetum andDiprion similis on white pine (1988)
    Springer
    Journal of chemical ecology 14 (1988), S. 1131-1144 
    Details
    ISSN: 1573-1561
    Keywords: Sawflies ; Neodiprion pinetum ; Diprion similis ; Hymenoptera ; Diprionidae ; 3,7-dimethylpentadecan-2-ol ; Pinus strobus ; sex pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex pheromones ofNeodiprion pinetum (Norton) andDiprion similis (Hartig) consist of two isomers, 2S,3S,7S and 2S,3R,7R, in either the acetate orpropionate forms of 3,7-dimethylpentadecan-2-ol, respectively. The 2S,3S,7S acetate isomer is utilized byN. pinetum as the major pheromone component and the 2S,3R,7R acetate as a Synergist. ConverselyD. similis utilizes 2S,3R,7R as propionate the major pheromone component and 2S,3S,7S propionate as a Synergist. This was confirmed in the field in both Michigan and Wisconsin. Capillary gas-liquid chromatographie analyses revealed that these two isomers are present in the natural pheromones of both species at the ratios close to those predicted by artificial blending of the two optical isomers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01019341
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    Paper (German National Licenses)
    Fulltext
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