ISSN:
1617-4623
Keywords:
Yolk protein
;
Ovarian enhancer 1
;
Enhancer-binding factors
;
Drosophila melanogaster
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract It has been reported that three different DNA regions — the fat body enhancer and ovarian enhancers 1 and 2 — direct the tissue-specific expression ofyp1 andyp2 inDrosophila melanogaster. In the present study, we identified ovarian enhancer 1-specific binding proteins. Electrophoretic mobility shift assay revealed that these proteins are present in the adult ovary, but not in adult testis or fat body. Southwestern blot analysis showed that about 130 kDa and 40 kDa proteins, designated OEF1 and OEF2, respectively, from ovarian nuclear or crude extracts bind specifically to the ovarian enhancer 1. The two proteins were partially purified by streptavidin/agarose-DNA affinity chromatography, and their binding activity was confirmed by electrophoretic mobility shift assay. These ovarian enhancer factors may play an important role in the regulation of transcription ofyp1 andyp2 in the ovary.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02172525
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