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  • Electronic Resource  (4)
  • ELISA  (2)
  • Pollination  (2)
  • detection  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Studies on flower longevity in Digitalis (1979)
    Springer
    Planta 146 (1979), S. 409-414 
    Details
    ISSN: 1432-2048
    Keywords: Corolla abscission ; Digitalis ; Flower longevity ; Pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Flower lifespan was terminated by corolla abscission 5–6 days after stigma opening in the unpollinated flower. Increased pollen loads produced increased seed set and reduced flower longevity progressively to a minimum of one day after pollination with pure pollen. Weakening of the abscission zone was detectable 8 h after pollination, whilst the pollen tubes were still within the stigmatic zone, suggesting that a stimulus, moving at 4 mm h−1 minimum, was transmitted through the style and ovary. Soon after pollination removal of the stigma prevented the pollination-induced corolla abscission. Later it was necessary to remove the stigma and upper style, and later still the whole style to delay abscission. The progressive induction of the stigma and style took place at a rate of 1.5 mm h−1, in advance of the pollen tubes which grew at 0.75 mm h−1. It was not possible to reproduce the pollination effects by application of indoleacetic acid (IAA) to the stigma or the style.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380853
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on flower longevity in Digitalis (1983)
    Springer
    Planta 157 (1983), S. 15-21 
    Details
    ISSN: 1432-2048
    Keywords: Abscission ; Corolla (abscission) ; Digitalis ; Ethylene and abscission ; Pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The flowers of Digitalis purpurea respond to pollination by rapid corolla abscission without any loss of corolla turgor, nor any significant loss of corolla constituents, relative to the corollas of unpollinated flowers of a similar age. The corollas of unpollinated flowers too eventually abscise, 6 d after the stigma opens, however, they do so with only a minimal loss of fresh weight or corolla constituents. Pollination causes an increase in ethylene production detectable within 1 h. Increased ethylene production occurs initially only from the upper portion of the style, later from the lower portion, and lastly, between 23 and 48 h after pollination, from the ovary plus calyx. The pollination response can be induced by exogenous ethylene, the degree of weakening of the corolla abscission zone being dependent upon the concentration and duration of the exposure period and on the stage of flower development. The regulation of ethylene biosynthesis and its involvement in the control of pollination-induced corolla abscission are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394535
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers grown from seed pieces with various levels of inoculum (1992)
    Springer
    Potato research 35 (1992), S. 207-216 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was used to monitor potato stems of three cultivars for the presence of the ring rot pathogen,Clavibacter michiganensis subsp.sepedonicus, during the growing season and in progeny tubers after harvest. The highest ELISA values were obtained with the highest concentration of bacteria used to inoculate seed pieces in all cultivars tested. Low ELISA values were obtained for stems and progeny tubers selected from plants grown from seed inoculated at lower bacterial concentrations. Estimates of bacterial densities in stems and progeny tubers by immunofluorescence indicated that low ELISA values were most probably caused by low bacterial numbers. It is suggested that the sensitivity of ELISA for detecting the ring rot pathogen in potato stems and progeny tubers is a function of the concentration of bacteria in individual seed pieces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357617
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  • 4
    Electronic Resource
    Electronic Resource
    A comparative study in five laboratories on detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers (1992)
    Springer
    Potato research 35 (1992), S. 217-226 
    Details
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; eggplant ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato stems and tubers grown in the field from seed tubers inoculated withClavibacter michiganensis subsp.sepedonicus, which causes bacterial ring rot, were tested by indirect, sandwich enzyme-linked immunosorbent assay (ELISA) in five laboratories. Correlation between values for each experimental treatment from the five laboratories was greater (r=0.86) than correlation between values for individual samples (r=0.71). When three or more laboratories obtained ELISA values of ≥0.200 for a sample, that sample was presumed to be positive. Conversely, when three or more laboratories obtained ELISA values 〈0.200, the consensus determination was regarded as negative. The percentage of stem and tuber samples that were in agreement with the consensus ELISA determination varied from 65.5 to 96.7%. Indirect immunofluorescence tests, conducted on the same samples in two laboratories, were consistent with 83.4–91.9% of the consensus ELISA determinations. Presence or absence ofC.m. sepedonicus was confirmed in some samples by an eggplant bioassay and direct isolatiion of the bacterium. The ELISA procedure was well suited for screening large numbers of samples and this study confirms it to be a promising procedure in routine indexing of seed potatoes forC.m. sepedonicus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357618
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    Paper (German National Licenses)
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