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Gas vesicles are strengthened by the outer-surface protein, GvpC (1992)

Hayes, P. K. ; Buchholz, B. ; Walsby, A. E.

Springer

Archives of microbiology 157 (1992), S. 229-234

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ISSN: 1432-072X

Keywords: Cyanobacteria ; Gas vesicles ; Protein reassembly ; Anabaena flos-aquae

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The critical collapse pressure of gas vesicles isolated from Anabaena flos-aquae decreased from 0.557 to 0.190 MPa when GvpC, the hydrophilic 22 kDa protein present on the outer surface of the gas vesicle, was removed by rising in 6 M urea. Recombinant GvpC was purified from inclusion bodies, produced in an E. coli strain containing an expression vector bearing the gene ecoding GvpC from A. flos-aquae, and then solubilised in 6 M urea. This recombinant GvpC became bound to gas vesicles that had been stripped of their native protein, when the urea was removed by dialysis; the amount which bound increased with the concentration of GvpC present. The critical pressure of these reconstituted gas vesicles increased to 0.533 MPa, 96% of the original value. These results indicate that the function of GvpC is to increase the strength of the structure.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00245155

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139. Verbesserung der Organqualität bei der Nierenspende (1981)

Buchholz, B. ; Zastrow, F. ; Lison, A. E.

Springer

Langenbeck's archives of surgery 355 (1981), S. 530-530

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ISSN: 1435-2451

Keywords: Kidney donation ; Donor procurement ; En-bloc perfusion ; Nierenspende ; organerhaltende Therapie ; En-bloc Perfusion

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Description / Table of Contents: Zusammenfassung Neben immunologischen Faktoren nimmt die Organqualität bei der Nierenspende eine Schlüsselposition für den Transplantationserfolg ein. Anhand von 110 Organentnahmen werden die wesentlichen organisatorischen und intensivmedizinischen Maßnahmen sowie die in Münster weiterentwickelte extracorporale transaortale Nierenperfusion dargestellt.

Notes: Summary Besides immunological problems the anatomical and functional integrity of the transplanted kidney has a decisive influence on the outcome of kidney grafting. Using the data obtained from 110 kidneys explanted in Münster, we outline the essential regimen of donor procurement in an intensive care unit. A sophisticated technique in terms of extracorporeal transaortic en bloc graft perfusion is offered.