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  • Electronic Resource  (2)
  • Engineering General  (1)
  • Graphical genotyping  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 1133-1144 
    ISSN: 1432-2242
    Keywords: Key wordsLycopersicon esculentum ; L. pimpinellifolium ; Salt tolerance ; Restriction fragment length polymorphism (RFLP) ; Quantitative trait loci (QTLs) ; Seed germination ; Molecular markers ; Graphical genotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Most cultivars of tomato (Lycopersicon esculentum) are sensitive to salinity during seed germination and at later stages. Genetic resources for salt tolerance have been identified within the related wild species of tomato. The purpose of the present study was to identify quantitative trait loci (QTLs) for salt tolerance during germination in an inbred backcross (BC1S1) population of an interspecific cross between a salt-sensitive tomato breeding line (NC84173, maternal and recurrent parent) and a salt-tolerant Lycopersicon pimpinellifolium accession (LA722). Onehundred and nineteen BC1 individuals were genotyped for 151 restriction fragment length polymorphism (RFLP) markers and a genetic linkage map was constructed. The parental lines and 119 BC1S1 families (self-pollinated progeny of 119 BC1 individuals) were evaluated for germination at an intermediate salt-stress level (150 mM NaCl+15 mM CaCl2, water potential approximately −850 kPa). Germination was scored visually as radicle protrusion at 8-h intervals for 28 consecutive days. Germination response was analyzed by survival analysis and the time to 25, 50, and 75% germination was determined. In addition, a germination index (GI) was calculated as the weighted mean of the time from imbibition to germination for each family/line. Interval mapping, single-marker analysis and distributional extreme analysis, were used to identify QTLs and the results of all three mapping methods were generally similar. Seven chromosomal locations with significant effects on salt tolerance were identified. The L. pimpinellifolium accession had favorable QTL alleles at six locations. The percentage of phenotypic variation explained (PVE) by individual QTLs ranged from 6.5 to 15.6%. Multilocus analysis indicated that the cumulative action of all significant QTLs accounted for 44.5% of the total phenotypic variance. A total of 12 pairwise epistatic interactions were identified, including four between QTL-linked and QTL-unlinked regions and eight between QTL-unlinked regions. Transgressive phenotypes were observed in the direction of salt sensitivity. The graphical genotyping indicated a high correspondence between the phenotypes of the extreme families and their QTL genotypes. The results indicate that tomato salt tolerance during germination can be improved by marker-assisted selection using interspecific variation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 18 (1994), S. 863-880 
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A direct time-domain numerical procedure is proposed to analyse the transient dynamic response of two-dimensional reservoir-dam-soil systems. The reservoir extends to infinity and the dam is supported by an unbounded soil. The structure with either linear or non-linear material properties is modelled by the Finite Element Method (FEM). The soil is assumed to be an elastic, isotropic and homogeneous half-space represented by a boundary condition in the form of generalized impedance determined by the transient Lamb's solution due to a uniformly distributed traction imposed on the free surface, Guan and Novak.1 Moreover, a technique is developed to include the influence of the reservoir on the dam in terms of nodal accelerations along their interface at different time steps. The advantages of the proposed procedure are obvious. For example, it avoids any additional discretization of the boundaries except the soil-dam interface, and the influence matrix of the fluid is obtained explicitly using shape functions defined at the upstream face of the dam without the finite analysis of the reservoir so that it works very efficiently. Numerical results for a system consisting of reservoir, elastic dam and foundation subjected to the San Fernando, 1971 earthquake ground motion are presented.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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