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  • Electronic Resource  (2)
  • Engineering General  (1)
  • RNA quasi-species  (1)
  • 1
    ISSN: 1572-994X
    Keywords: viroids ; apscaviroids ; phylogenetic analysis ; RNA quasi-species ; subviral RNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Field-grown citrus trees often harbor complex mixtures of 4–5 different viroid species, and the presence of citrus viroid III (CVd-III) has been shown to reduce the rate of tree growth without inducing disease. To more fully define the structure of its quasi-species, we have examined nine citrus viroid complexes for the presence of previously undescribed sequence variants of CVd-III. Analysis of 86 full-length cDNAs generated from these nine viroid complexes by RT-PCR revealed the presence of 20 new CVd-III variants. Chain lengths ranged from 293–297 nucleotides, and sequence changes were confined largely to the lower portions of the central conserved region and variable domain. The previously described variants CVd-IIIa (297 nt) and CVd-IIIb (294 nt) were clearly predominant, but phylogenetic analysis indicated that certain isolates may contain representatives of two additional fitness peaks. At least one group of CVd-III variants appears to have arisen as a result of RNA recombination. Populations recovered from diseased/declining trees were the most diverse, but even dwarfing isolates originating from old line Shamouti trees showed considerable variability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 1517-1534 
    ISSN: 0029-5981
    Keywords: spectral elements ; viscoelasticity ; time-splitting method ; drag factor ; Uzawa algorithm ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The steady flow of a viscoelastic fluid past a sphere in a cylindrical tube is considered. A spectral element method is used to solve the system of coupled non-linear partial differential equations governing the flow. The spectral element method combines the flexibility of the traditional finite element method with the accuracy of spectral methods. A time-splitting algorithm is used to determine the solution to the steady problem. Results are presented for the Oldroyd B model. These show excellent agreement with the literature. The results converge with mesh refinement. A limiting Deborah number of approximately 0⋅6 is found, irrespective of the spatial resolution.
    Additional Material: 25 Ill.
    Type of Medium: Electronic Resource
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