ISSN:
1432-072X
Keywords:
Key words Polygalacturonase
;
Saccharomyces
;
cerevisiae
;
Genetic determination
;
Mutants
;
Complementation groups
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The genetic determination of polygalacturonase (PG) production in Saccharomyces cerevisiae was studied by biochemical and classical genetic techniques. Crosses of PG+ strains with PG– strains showed that in the haploid wild-type-derived strain, two structural genes were involved in the production of a hydrolysis halo on plates with polygalacturonic acid. However, in the case of PG+ laboratory strain IM1-8b, the phenotype was controlled by only one structural gene although the analysis of PG– IM1-8b mutants demonstrated the existence of at least two complementation groups. All these genetic results were assessed biochemically by means of cation-exchange chromatography. Two enzymes were separated in the wild-type strain, and only one in the laboratory strain. The three enzymes had different K m values, molecular masses, and optimal pHs for activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050445
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