ISSN:
0170-2041
Keywords:
Carbohydrates
;
Oligosaccharides
;
Saccharides
;
Glycoproteins
;
Glycosyltransferase
;
Transferases
;
Enzymes
;
Proteins
;
Trichloroacetimidates
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Building Units of Oligosaccharides, CVII. - Synthesis of Modified Oligosaccharides of N-Glycoproteins for Substrate Specificity Studies of N-Acetylglucosaminyltransferase IISeveral modified derivatives of the tetrasaccharide β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→6)]-β-D-Man-octyl (1) were synthesized for substrate specifity studies of N-acetylglucosaminyltransferase II (GlcNAcT II). The hydroxyl groups at C-3, C-4 or C-6 of the α(1→6)-linked Man residue and the hydroxyl groups at C-3 and C-6 of the α(1→3)-linked Man were replaced by deoxy groups. All five tetrasaccharides were synthesized by a block synthesis by using deoxy saccharide residues. The trichloroacetimidate method was particulary successful for synthesizing the glycosidic linkages. The modified compounds have been tested as substrates for N-acetylglucosaminyltransferase II (GlcNAcT II) from rat liver. The substrate specificity of GlcNAcT II will be discussed.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jlac.1993199301118
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