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  • Electronic Resource  (7)
  • Granule-bound starch synthase  (4)
  • Solanum  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Expression patterns of two starch biosynthetic genes in in vitro cultured cassava plants and their induction by sugars
    Amsterdam : Elsevier
    Plant Science 98 (1994), S. 53-62 
    Details
    ISSN: 0168-9452
    Keywords: Branching enzyme ; Cassava ; Expression patterns ; Granule-bound starch synthase ; Manihot esculenta Crantz ; Sugar incubation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(94)90147-3
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.) (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 217-221 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Mutant ; Starch composition ; Granule-bound starch synthase ; amylose-free
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An amylose-free potato mutant was isolated after screening 12,000 minitubers. These minitubers had been induced on stem segments of adventitious shoots, which had been regenerated on leaf explants of a monoploid potato clone after Röntgen-irradiation. The mutant character is also expressed in subterranean tubers and in microspores. Starch granules from the mutant showed a strongly reduced activity of the granule bound starch synthase and loss of the major 60 kd protein from the starch granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249167
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  • 3
    Electronic Resource
    Electronic Resource
    Relative performance of monohaploid potato clones and their diploid parents at plant level and after protoplast isolation and subsequent fusion (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 451-458 
    Details
    ISSN: 1432-2242
    Keywords: Monohaploids ; Solanum ; Potato ; In vitro-performance ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plant growth performance was studied in 118 potato monohaploids and in their diploid parents. Of these monohaploids 76 were also investigated at the protoplast level and eight of these were used in protoplast fusion experiments as well. No correlation was found between relative performance of greenhouse grown and in vitro grown plants. No or only weak correlations were found between different in vitro characteristics such as plant growth, protoplast yield per gram plant material, plating efficiency and callus growth. This indicates the unpredictability of these characters. The protoplast fusion experiments indicated that only in some genotype combinations increased callus growth rates may be found. However, it is not clear whether such calli were hybrids or not. In protoplast monocultures only diploid and tetraploid regenerants were obtained. After fusion, tetraploids but also some triploids could be regenerated. The finding of triploids indicates that monoploid protoplasts were involved in fusion. Isozyme analysis and morphological assessment of the plants pointed out that the majority of the fusion regenerants were hybrids. The implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289820
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of a wild-type GBSS gene introduced into an amylose-free potato mutant by Agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 369-375 
    Details
    ISSN: 1432-2242
    Keywords: Complementation ; Granule-bound starch synthase ; Amylose content ; Gene expression ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Transformation of a diploid amylose-free (amf) potato mutant with the gene encoding GBSS leads to the restoration of amylose synthesis. Transformants were obtained which had wild-type levels of both GBSS activity and amylose content. It proved to be difficult to increase the amylose content above that of the wild-type potato by the introduction of additional copies of the wild-type GBSS gene. Staining of starch with iodine was suitable for investigating the degree of expression of the inserted GBSS gene in transgenic amf plants. Of the 19 investigated transformants, four had only red-staining starch in tubers indicating that no complementation of the amf mutation had occured. Fifteen complemented transformants had only blue-staining starch in tubers or tubers of different staining categories (blue, mixed and red), caused either by full or partial expression of the inserted gene. Complementation was also found in the microspores. The segregation of blue- and red-staining microspores was used to analyse the inheritance of the introduced GBSS genes. A comparison of the results from microspore staining and Southern hybridisation indicated that, in three tetraploid transgenics, the gene was probably inserted before (duplex), and in all others after, chromosome doubling (simplex). The partial complementation was not due to methylation of the HPAII/MSPI site in the promoter region. Partially complemented plants had low levels of mRNA as was found when the GBSS expression levels were inhibited by anti-sense technology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223647
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  • 5
    Electronic Resource
    Electronic Resource
    A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in potato (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 654-661 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; CAPS ; G. pallida ; G. rostochiensis ; Solanum ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Broad-spectrum resistance in potato to the potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida is commonly regarded as a polygenically inherited trait. Yet, by use of QTL analysis and a selected set of PCN populations, resistance to both PCN species could be ascribed to the action of locus Grp1. Grp1 confers major resistance to G. rostochiensis line Ro5-22 and G. pallida population Pa2-D383 and partial resistance to G. pallida population Pa3-Rookmaker. Grp1 was mapped on chromosome 5 using previously characterized AFLP markers. Cleaved amplified polymorphic sequence (CAPS) markers available for RFLP loci GP21 and GP179 revealed that Grp1 maps on a genomic region harboring other resistance factors to viral, fungal and nematodal pathogens. The present data indicate that Grp1 is a compound locus which contains multiple genes involved in PCN resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050785
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  • 6
    Electronic Resource
    Electronic Resource
    Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs (1991)
    Springer
    Molecular genetics and genomics 225 (1991), S. 289-296 
    Details
    ISSN: 1617-4623
    Keywords: Amylose content ; Antisense RNA ; Dominant (hemizygous) suppression ; Granule-bound starch synthase ; Transgenic potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Granule-bound starch synthase [GBSS; EC 24.1.21] determines the presence of amylose in reserve starches. Potato plants were transformed to produce antisense RNA from a gene construct containing a full-length granule-bound starch synthase cDNA in reverse orientation, fused between the cauliflower mosaic virus 35S promoter and the nopaline synthase terminator. The construct was integrated into the potato genome by Agrobacterium rhizogenes-mediated transformation. Inhibition of GBSS activity in potato tuber starch was found to vary from 70% to 100%. In those cases where total suppression of GBSS activity was found both GBSS protein and amylose were absent, giving rise to tubers containing amylose-free starch. The variable response of the transformed plants indicates that position effects on the integrated sequences might be important. The results clearly demonstrate that in tubers of potato plants which constitutively synthesize antisense RNA the starch composition is altered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269861
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of parental effects on mean vine maturity and chip colour of 4x.2x potato progenies (1996)
    Springer
    Euphytica 88 (1996), S. 175-179 
    Details
    ISSN: 1573-5060
    Keywords: chip colour ; diploids ; sexual polyploidization ; Solanum ; vine maturity ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The parental effects on progenies from potato 4x.2x(FDR) crosses were analyzed to determine anether and to what extent selection criteria used for selecting tetraploid breeding lines have to be adjusted when selecting diploid breeding lines. For vine maturity and chip colour multiple regression analysis of progeny means on means of diploid and tetraploid parents revealed high coefficients of determination (R2) for both characters, indicating good predicting power of the performance of both diploid and tetraploid parents on the performance of their 4x.2x progenies. For vine maturity the multiple regression slope for the tetraploid perents was significantly larger than for the diploid parents, and progeny means were towards the tetraploid parents. This indicates that selection criteria for vine maturity may be less stringent at the diploid level. However, diploids may be earlier maturing than tetraploids and therefore selection criteria can better be similar at both ploidy levels. For chip colour, multiple regression slopes for diploid and tetraploid parents were similar and progeny means were towards the darkest coloured parent irrespective of its ploidy level. This indicates that selection of breeding lines for chip colour can be conducted similarly at both ploidy levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023888
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