ISSN:
1572-994X
Keywords:
HSV-1 thymidine kinase
;
site-directed mutagenesis
;
HSV-1 TK antiserum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The thymidine kinase (TK) of herpes simplex virus type 1 (HSV-1) contains three regions of homology to other ATP utilizing enzymes. We have altered one region of the protein, which seems to play an important role in phosphorylating substrates by site-directed mutagenesis. When the aspartate 162 was changed to asparagine, the enzyme lost its activity. To identify the inactive protein, expressed by a vaccinia vector in eukaryotic cells, a monospecific antiserum against a bacterial tryptophan E-HSV-1 TK fusion protein was made. These results support the suggestion that aspartate 162 is essential for the enzymatic activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01702400
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