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  • Electronic Resource  (12)
  • Hordeum bulbosum  (9)
  • Plasma  (3)
  • bulbous barley grass  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Studies of rat alkaline phosphatase (1978)
    Springer
    Archives of toxicology 39 (1978), S. 267-287 
    Details
    ISSN: 1432-0738
    Keywords: Rat ; Plasma ; Alkaline phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes the application of a test system which applies different substrates, inhibitors and acrylamide electrophoresis to the study of the alkaline phosphatase activity of rats. The system, which was specifically devised to characterise the alkaline phosphatase activity of small quantities of plasma from rats in toxicological studies, was also used to examine the plasma from rats under several physiological conditions, e.g. time of day, age, diet, etc. These physiological factors can influence the measurement of plasma alkaline phosphatase activity and should be taken into account when designing toxicological studies. Different experimental regimes were used to produce increases in liver, bone and intestinal alkaline phosphatase isoenzymes in the plasma of rats and these were reflected in the characterisations by the system. Although much remains unknown, a few concepts of rat plasma alkaline phosphatase activity emerged under our conditions. There is a ‘tidal’ intestinal component which arises from feeding and is approximately 60% of the total activity of the fed rat. In the fasted rat this ‘tidal’ component has largely disappeared and the ‘core’ activity which remains is composed of approximately equal proportions of bone isoenzyme and another as yet ill-defined component. The liver isoenzyme which appears in the plasma of the cholestatic rat appears to be either a very minor component or absent from the plasma of our normal CFE derived rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296387
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Methods for the estimation of acetylcholinesterase activity in the plasma and brain of laboratory animals given carbamates or organophosphorus compounds (1971)
    Springer
    Archives of toxicology 27 (1971), S. 292-310 
    Details
    ISSN: 1432-0738
    Keywords: Cholinesterase ; Plasma ; Brain ; Carbamate ; Organophosphorus Compounds ; Cholinesterase ; Plasma ; Gehirn ; Carbamate ; Organophosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Dieser Report beschreibt die Modifikationen, die notwendig sind, um die pH-Stat und Ellman-Methode für die Bestimmung der Cholinesteraseaktivität im Plasma und Gehirn der verschiedenen Laboratoriumstierarten anzupassen. Beide Methoden wurden durch Untersuchungen bewertet, bei denen den Tieren cholinesterasehemmende Stoffe, Carbamate und organische Phosphorverbindungen, gegeben worden waren.
    Notes: Abstract This report describes the modifications necessary to adapt the pH Stat and Ellman methods for the estimation of cholinesterase activity in the plasma and brain of various species of laboratory animals. Both methods were evaluated in studies in which cholinesterase inhibitors, carbamates and organophosphorus compounds, were given to the animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315051
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  • 3
    Electronic Resource
    Electronic Resource
    Studies of rat alkaline phosphatase (1978)
    Springer
    Archives of toxicology 39 (1978), S. 249-266 
    Details
    ISSN: 1432-0738
    Keywords: Rat ; Alkaline phosphatase ; Plasma ; Organs ; Toxicity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A manual system of various estimations of rat plasma alkaline phosphatase activity has been devised for small volumes of plasma which uses different inhibitors, compares the utilisation of two substrates and includes acrylamide gel electrophoresis. The different inhibitors etc. allow a degree of discrimination between alkaline phosphatase extracts of rat organs. The properties of isoenzymes, e.g. intestinal alkaline phosphatase, differ depending upon the environment in which they are studied. In conjunction, if necessary, with the methods described for the estimation of liver and intestinal alkaline phosphatase activity, it is hoped to use the system to discriminate between the isoenzymes present in the plasma alkaline phosphatase of rats in toxicological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296386
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  • 4
    Electronic Resource
    Electronic Resource
    Locating introgressions of Hordeum bulbosum chromatin within the H. vulgare genome (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 27-31 
    Details
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare ; Hordeum bulbosum ; Leaf rust resistance ; Gene introgression ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00002904
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  • 5
    Electronic Resource
    Electronic Resource
    Characterisation of progeny from backcrosses of triploid hybrids between Hordeum vulgare L. (2x) and H. bulbosum L (4x) to H. vulgare (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 460-464 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum bulbosum ; Fertile triploids ; Gene introgression ; Chromosome substitutions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific hybridisations between Hordeum vulgare L. (cultivated barley) and H. bulbosum L. (bulbous barley grass) have been carried out to transfer desirable traits, such as disease resistance, from the wild species into barley. In this paper we report the results of an extensive backcrossing programme of triploid hybrids (H. vulgare 2x x H. bulbosum 4x) to two cultivars of H. vulgare. Progenies were characterised cytologically and by restriction fragment length polymorphism analysis and comprised (1) haploid and diploid H. vulgare plants, (2) hybrids and aneuploids, (3) single and double monosomic substitutions of H. bulbosum chromosomes into H. vulgare and (4) chromosomal rearrangements and recombinants. Five out of the seven possible single monosomic chromosome substitutions have now been identified amongst backcross progeny and will be valuable for directed gene introgression and genome homoeology studies. The presence amongst progeny of 1 plant with an H. vulgare-H. bulbosum translocated chromosome and one recombinant indicates the value of fertile triploid hybrids for interspecific gene introgression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223661
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  • 6
    Electronic Resource
    Electronic Resource
    The transfer of a powdery mildew resistance gene from Hordeum bulbosum L to barley (H. vulgare L.) chromosome 2 (2I) (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1288-1292 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum bulbosum ; Powdery mildew resistance ; Gene introgression ; Irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hordeum bulbosum L. is a source of disease resistance genes that would be worthwhile transferring to barley (H. vulgare L.). To achieve this objective, selfed seed from a tetraploid H. vulgare x H. bulbosum hybrid was irradiated. Subsequently, a powdery mildew-resistant selection of barley phenotype (81882/83) was identified among field-grown progeny. Using molecular analyses, we have established that the H. bulbosum DNA containing the powdery mildew resistance gene had been introgressed into 81882/83 and is located on chromosome 2 (2I). Resistant plants have been backcrossed to barley to remove the adverse effects of a linked factor conditioning triploid seed formation, but there remains an association between powdery mildew resistance and non-pathogenic necrotic leaf blotching. The dominant resistance gene is allelic to a gene transferred from H. bulbosum by co-workers in Germany, but non-allelic to all other known powdery mildew resistance genes in barley. We propose Mlhb as a gene symbol for this resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220943
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of pollination bag type on seed quality and size in Hordeum inter- and intraspecific hybridization (1982)
    Springer
    Euphytica 31 (1982), S. 439-449 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barleygrass ; seed quality ; crossing bag
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of different spike covering materials have been investigated in crosses between Hordeum vulgare and H. bulbosum and in selfed H. vulgare. It was found that after lemmas and paleas were clipped, improvements in seed quality (H. vulgare × H. bulbosum) and weight (selfed H. vulgare) were obtained by covering heads with small brown paper bags compared with other treatments. Possible reasons for these effects are discussed but so far the mechanism has not been clarified. However, light is suggested as playing a major role.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021661
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  • 8
    Electronic Resource
    Electronic Resource
    The influence of genotype on doubled haploid barley production (1983)
    Springer
    Euphytica 32 (1983), S. 863-876 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barleygrass ; haploid embryos ; doubled haploids ; genotype influence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between five cultivars of Hordeum vulgare and three genotypes of H. bulbosum and the influence of male and female partners on success rates up to the stage of haploid embryo culture was investigated. Both parents markedly affected seed setting and rates of embryo differentiation, whereas seed quality was mainly influenced by the female. There was an interaction between certain genotypes when overall embryo culture rates were analysed. Following embryo culture only the influence of the female partner was assessed but differences were found between the H. vulgare cultivars regarding rates of hybrid (VB) production and chromosome doubling whereas total plant regeneration was unaffected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042168
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  • 9
    Electronic Resource
    Electronic Resource
    Attempts to overcome partial incompatibility between Hordeum vulgare L. and H. bulbosum L. (1980)
    Springer
    Euphytica 29 (1980), S. 369-377 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; interspecific cross ; incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Partial incompatibility has already been reported between Hordeum vulgare and H. bulbosum during the course of doubled haploid production, and in this paper attempts to overcome the breeding barrier are described. The methods which seem to offer most success are those of environmental adjustments and the adoption of new genotypes of H. bulbosum. Further cultivars of H. vulgare exhibiting this phenomenon are also noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025135
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  • 10
    Electronic Resource
    Electronic Resource
    Plant regeneration from cultured embryos derived from Hordeum vulgare L. Pollinated with H. Bulbosum L. (1983)
    Springer
    Euphytica 32 (1983), S. 585-591 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; embryo culture ; haploids ; hybrids ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant regeneration rates from embryos derived from Hordeum vulgare x H. bulbosum were recorded over a 3 1/2 year period. % total plant regeneration (haploids + VB hybrids) varied to some extent but did not seem to be influenced by season or male parent. % VB production (and thus chromosome elimination) was however markedly affected by season and the genotype of the pollinator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021471
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