ISSN:
1432-2013
Keywords:
Key words Type-I collagen
;
Insulin-like growth factor-I
;
SaOS-2 osteoblast-like cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The effect of insulin-like growth factor-I on collagen synthesis was studied using cultured human osteoblast-like SaOS-2 cells by measuring the incorporation of tritiated L-proline into immunoprecipitable type-I collagen. Tritiated L-proline incorporation into collagen was significantly stimulated by insulin-like growth factor-I in a time- and concentration-dependent manner. Unlabelled L-proline and α-(methylamino) isobutyric acid inhibited either the influx into cells, or the incorporation into collagen, of tritiated L-proline. The increase in incorporation of tritiated L-proline was significantly reduced by cycloheximide and actinomycin D. L-Proline incorporation into collagen was also stimulated by insulin-like growth factor-II, insulin-like growth factor-I analogues and insulin. The insulin-like growth factor-I-stimulated L-proline incorporation was inhibited by one of its binding proteins, insulin-like growth factor binding protein-4, in a concentration-dependent manner.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050257
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