ISSN:
1573-904X
Keywords:
organic cation
;
epithelial transport
;
LLC-PK1
;
kinetic model
;
levofloxacin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. The aims of this study were to establish a kinetic means of analyzing the membrane transport of organic cations in renal epithelial cells, and to simultaneously evaluate drug interactions in apical and basolateral membranes. Methods. Tetraethylammonium (TEA) transport was measured using LLC-PK1, cell monolayers grown on microporous membrane filters. After incubating the cells with unlabeled TEA or other drugs, apical or basolateral medium was changed to that containing labeled TEA, and transcellular transport and cellular accumulation were measured. Clearance from apical medium to cells (CL12), cells to apical medium (CL21), cells to basolateral medium (CL23) and basolateral medium to cells (CL32) were calculated based on a three compartment model. Results. TEA was accumulated progressively in the monolayers from the basolateral side and was transported unidirectionally to the apical side. CL32 was greater than CL12 and CL23 was greater than CL21. Therefore, the rate limiting step of TEA transport from the basolateral to the apical medium was the cell-to-apical step. Co-incubation of TEA with procainamide decreased the transport parameters of TEA, CL12, CL21 and CL32, whereas that with levofloxacin decreased only CL12 and CL21, not affecting the parameters in basolateral membranes. Conclusions. Using a simple model, we analyzed the transport of organic cation in kidney epithelial cell line, LLC-PK1 This method can be useful for the analysis of cation transport and drug interactions in the apical and basolateral membranes of renal tubules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1012119210434
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