ISSN:
1432-2013
Keywords:
Thrombin
;
Xenopus laevis
;
oocytes
;
osmosensitivity
;
follicular cells
;
PKC
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The presence and properties of thrombin receptors have been investigated inXenopus laevis follicles. In follicles, voltage-clamped at -60 mV or -80 mV, sub-micromolar concentrations of thrombin (Thr) induce smooth inward currents carried by chloride ions, which desensitize over tens of minutes. This desensitization is prevented by PKC inhibitors (staurosporine and H7). Responses are inhibited by hirudin. Inconsistent responses can be observed in defolliculated oocytes. Tbr responses, readily recorded in hypo-osmotic solution are abolished by perfusion of normal frog “Ringer” or by the addition of 50 mM sucrose. Thus Thr acts on receptors present in the follicular cells triggering the opening of chloride channels by acting on receptors, likely to be located on follicular cells, via a PKC-regulated Pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02346356
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