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  • 1
    Electronic Resource
    Electronic Resource
    Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping (1984)
    Springer
    International journal of legal medicine 93 (1984), S. 259-268 
    Details
    ISSN: 1437-1596
    Keywords: Monoclonal antibody ; AB0 system, bloodstains ; Absorption inhibition, bloodstains ; Absorption elution, bloodstains ; Blutgruppen, monoklonale Antikörper ; Anti-H, Ulex-Präparate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Zwei verschiedene monoklonale Anti-A- und Anti-B-Reagenzien sowie verschiedene Lectin-I-Präparate, durch Affinitätschromatographie aus Ulex-europaeus-Samen gereinigt, wurden mit üblichen Anti-A- und Anti-B-Seren sowie Ulex-Anti-H hinsichtlich ihrer serologischen Eigenschaften im Hemmtest mit Sekretor-Speichel und im Elutionstest bei Blutspuren miteinander verglichen. Die monoklonalen Reagenzien und Ulex-Präparate sind mit den bisher üblichen Reagenzien durchaus vergleichbar und eignen sich gleichermaßen für forensische Hemm- und Elutionstests.
    Notes: Summary Two different monoclonal anti-A and anti-B and several different affinity purified Ulex europaeus lectin I reagents were evaluated and compared with conventional anti-A and anti-B sera and Ulex anti-H for serologic properties, in inhibition tests with secretor salivas, and in elution tests with bloodstains. The monoclonal and purified reagents were found to be comparable to conventional ones, and accordingly suitable for forensic inhibition and elution procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198651
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  • 2
    Electronic Resource
    Electronic Resource
    Production and purification of monoclonal antibodies to Pisum and Avena phytochrome (1983)
    Springer
    Planta 158 (1983), S. 369-376 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Monoclonal antibody ; Phytochrome (radioimmunoassay) ; Pisum (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract At least nine monoclonal antibodies against phytochrome from Pisum sativum L. and 20 against phytochrome from Avena sativa L. have been obtained from mouse hybridomas that were produced by fusion of spleen cells with SP 2/O-Ag14 myeloma cells. Hybridomas were selected and cloned in a single step by plating on a semisolid methylcellulose medium. Eight antibodies to Pisum and one to Avena phytochrome were immunopurified from hybridoma medium or ascitic fluid. When necessary, secreted antibodies were verified to be against phytochrome by demonstrating to be against phytochrome by demonstrating immunoadsorption of phytochrome, detected as loss of photoactivity and-or by appearance of the approx. 120,000-dalton phytochrome band upon sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397340
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  • 3
    Electronic Resource
    Electronic Resource
    Immunofluorescence visualization of phytochrome in Pisum sativum L. epicotyls using monoclonal antibodies (1983)
    Springer
    Planta 159 (1983), S. 545-553 
    Details
    ISSN: 1432-2048
    Keywords: Immunosorbent assay (phytochrome) ; Monoclonal antibody ; Phytochrome (immunofluorescence) ; Pisum (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the cellular distribution of phytochrome in epicotyls of dark-grown pea (Pisum sativum L.) seedlings using monoclonal antibodies to pea phytochrome. Screening of the eight available antibodies both by an enzymelinked immunosorbent assay (ELISA) and by their ability to visualize phytochrome in situ by immunocytochemical fluorescence demonstrated that: (1) three antibodies work well for immunofluorescence; (2) none of the eight antibodies discriminates between the red- and the far-red-absorbing forms of phytochrome (Pr, Pfr) as assayed by ELISA; (3) the antigenicity of phytochrome is reduced by fixation with formaldehyde with respect to all eight antibodies; and (4) two antibodies that bind well to formaldehyde-fixed phytochrome as assayed by ELISA do not bind well to phytochrome in situ. Phytochrome is observed in both cortical and stomatal guard cells of the epicotyl and exhibits a homogeneous cytoplasmic distribution in non-irradiated tissue. After red-light (R) treatment phytochrome becomes transiently inaccessible to antibodies. If maintained in the Pfr form for 10 min at room temperature before fixation, at least a portion of the phytochrome pool becomes accessible to antibodies and assumes a “sequestered” distribution. Both of these effects are almost entirely either prevented or reversed by subsequent far-red light treatment. We believe that the transient inaccessibility of phytochrome to antibodies after R irradiation is not a function of its conformational state. We suggest instead that R treatment rapidly induces an association of phytochrome with a subcellular component that interferes with antibody binding and that the “sequestered” areas represent a phytochrome pool that is distinct from both the diffusely distributed phytochrome in non-irradiated cells and from that phytochrome which is inaccessible to antibodies immediately after R irradiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409144
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  • 4
    Electronic Resource
    Electronic Resource
    Phytochrome quantitation in crude extracts of Avena by enzyme-linked immunosorbent assay with monoclonal antibodies (1983)
    Springer
    Planta 159 (1983), S. 534-544 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Enzyme-linked immunosorbent assay (phytochrome) ; Monoclonal antibody ; Phytochrome (immunoquantitation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An enzyme-linked immunosorbent assay (ELISA), which uses both rabbit polyclonal and mouse monoclonal antibodies to phytochrome, has been adapted for quantitation of phytochrome in crude plant extracts. The assay has a detection limit of about 100 pg phytochrome (〈1 fmol of monomer) and can be completed within 10 h. Nonspecific interference by crude plant extracts was detected and corrected for. Quantitation of phytochrome in crude extracts of etiolated oat (Avena sativa L.) seedlings by ELISA gave values that agreed well with those obtained by spectrophotometric assay. When etiolated oat seedlings were irradiated continuously for 24 h, the amount of phytochrome detected by ELISA and by spectrophotometric assay in crude extracts of these seedlings decreased by more than 1000-fold and about 100-fold, respectively. This discrepancy indicates that phytochrome in light-treated plants may be antigenically distinct from that found in fully etiolated plants. Both a decrease in the light and an increase in the dark of phytochrome content was observed in crude extracts of light-grown oat shoots, both green and Norflurazon-bleached, in response to a 12:12-h light-dark cycle. When these light-grown oat seedlings were kept in darkness for 48 h, phytochrome content detected by ELISA increased by 50-fold in crude extracts of green oat shoots, but only about 12-fold in extracts of herbicide-treated oat shoots. Phytochrome reaccumulation in green oat shoots was initially more rapid in the more mature cells of the primary leaf tip than near the basal part of the shoot. The inhibitory effect of Norflurazon on phytochrome accumulation was much more evident near the leaf tip than the shoot base. A 5-min red irradiation of oat seedlings at the end of a 48-h dark period resulted in a subsequent, massive decrease in phytochrome content in crude extracts from both green and Norflurazon-bleached oat shoots. These observations eliminate the possibility that substantial accumulation of chromophore-free phytochrome was being detected and indicate that Norflurazon has a substantial effect on phytochrome accumulation during a prolonged dark period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409143
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    Paper (German National Licenses)
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