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  • Electronic Resource  (2)
  • Nitrogen bases  (1)
  • plasmacytoma  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Growth and protein production kinetics of a murine myeloma cell line transfected with the human growth hormone gene (1991)
    Springer
    Cytotechnology 5 (1991), S. 223-231 
    Details
    ISSN: 1573-0778
    Keywords: cell culture ; kinetics ; Ig promoter/enhancer ; plasmacytoma ; recombinant protein production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A model mammalian cell system for the production of recombinant proteins was investigated. Murine myeloma cells which had lost the ability to produce both heavy and light chain immunoglobulin molecules were transfected with a vector containing the immunoglobulin heavy chain promoter and enhancer elements linked to the human growth hormone gene. The growth kinetics of G32, a clonal isolate, were found to be similar to both the parent myeloma and hybridomas. However, production of hGH by G32 was growth associated, rather than as a secondary metabolite as is the case for hybridomas. In addition, G32 produced hGH at molar levels greater than most hybridomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00556292
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The estimation of primary aromatic amines and other basic nitrogen compounds in some coal liquefaction products (1986)
    Springer
    Chromatographia 21 (1986), S. 67-76 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography-mass spectrometry ; Coal liquefaction products ; Nitrogen bases ; Selective derivatisation ; Quantitative analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The estimation of primary aromatic amines (PAA) and other basic nitrogen compounds in coal liquefaction products is described. Aqueous acid extraction was used to isolate basic nitrogen fractions which were analyzed by gas chromatography (GC) and mass spectrometry (MS). Selective chemical derivatization in conjunction with single-ion GC-MS was used to distinguish between the PAA and the isomeric aza heterocycles. The relative merits of the proprietary methylating reagent ‘Methyl-8’ (dimethylformamide dimethylacetal) and a more conventional fluoroacylating reagent for the selective derivatization of PAA are evaluated. Quantitative aspects of the analyses are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311678
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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