ISSN:
1432-072X
Keywords:
Escherichia coli
;
Phage Mu d, formic dehydrogenase
;
Seleno-polypeptide
;
Respiratory pathway
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The site of integration of phage Mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 kd) still able to incorporate Se. Synthesis of the truncated polypeptide is subject to the same regulatory signals as that of the wild-type enzyme. The formation of the 110 kd seleno-polypeptide, which is a constituent component of the formic dehydrogenase from the formate-nitrate respiratory pathway, is unimpaired in mutant M9s. The location of the gene for the 80 kd seleno-polypeptide was mapped at 92.4 min of the Escherichia coli chromosome.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00428850
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