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  • Electronic Resource  (5)
  • in vitro fertilization  (4)
  • Transmission electron microscopy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Centriolar dynamics in the bovine embryo: inheritance and perpetuation of the sperm centrosome during fertilization and development (1999)
    Springer
    Protoplasma 206 (1999), S. 263-269 
    Details
    ISSN: 1615-6102
    Keywords: Centriole ; Centrosome ; Cow ; Embryo ; Fertilization ; Transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of the centrosome (centriole) and its behaviour during fertilization and embryogenesis of cattle is presented. The bovine embryo follows the human pattern of centriole behaviour, which is common to most animals including large mammals. Thus, most mammals obey Boveri's rule of paternal centrosomal inheritance and perpetuation, whereas the mouse is an exception to the rule, showing maternal inheritance. The sperm centrosome was traced from fertilization to the hatching blastocyst stage in the cow and its presence was confirmed at every stage of cleavage, as reported in the human. It is concluded that the bovine embryo is a more appropriate model than the mouse for research in fertilization and assisted-reproduction technology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01288214
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis (1985)
    Springer
    Journal of assisted reproduction and genetics 2 (1985), S. 119-122 
    Details
    ISSN: 1573-7330
    Keywords: epididymis ; spermatozoa ; in vitro fertilization ; pregnancy ; sperm microaspiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Spermatozoa were collected by microaspiration from the corpus epididymidis of a 42-year-old man with secondary obstructive azoospermia and used for in vitro fertilization. At insemination 61% of the spermatozoa were motile, with a motility index of 157. One of five eggs was fertilized and this was subsequently transferred to the patient's wife at the two-cell stage. Ultrasound examination and changing hormone levels confirmed an ongoing pregnancy, which is currently at 30 weeks of gestation. This technique will provide a useful alternative for the management of some infertile men with obstructive azoospermia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01131497
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Support of the luteal phase in in vitro fertilization programs: Results of a controlled trial with intramuscular proluton (1985)
    Springer
    Journal of assisted reproduction and genetics 2 (1985), S. 166-169 
    Details
    ISSN: 1573-7330
    Keywords: luteal phase ; in vitro fertilization ; proluton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract There is disagreement among in vitro fertilization (IVF) programs as to the need to administer exogenous progesterone to support the luteal phase of patients undergoing embryo transfer after IVF. We examined the effect on pregnancy rates of Prolution, 50-mg daily injections given on days 7–16 following oocyte recovery, in 186 women undergoing IVF treatment using a combined stimulation regime of clomiphene and human menopausal gonadotropin (hMG). One group was deliberately selected for treatment on the possible criterion of lutealphase deficiency and two other groups were randomly selected into a treatment and a control group. No effect on pregnancy rate was noted in any of these groups. These results indicate that extension of the luteal phase with exogenous progesterone is unlikely to have a significant effect on increasing the pregnancy rate in IVF programs using similar treatment regimes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01131506
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  • 4
    Electronic Resource
    Electronic Resource
    The human pronuclear ovum: Fine strcture of monospermic and polyspermic fertilization in vitro (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 12 (1985), S. 385-398 
    Details
    ISSN: 0148-7280
    Keywords: in vitro fertilization ; pronuclear ova ; one-cell embryo ; monospermy ; polyspermy ; syngamy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The fine structure of pronuclear ova (monospermy and polyspermy) and one-cell embryos has been investigated in our IVF programme. Sixteen oocytes were collected at laparoscopy after appropriate hormonal stimulation and were matured and fertilized in vitro by methods that have given rise to normal pregnancies.Pronuclear ova showing monospermic fertilization had two vesicular pronuclei surrounded by aggregations of cellular organelles. The male pronucleus was closely associated with a sperm axoneme, while the female pronucleus was dismantling its envelope and condensing its chromatin ahead of its counterpart in late pronuclear ova. Each pronucleus had dispersed chromatin, dense compact nucleoli, and intranuclear annulate lamellae. Smooth endoplasmic reticulum, annulate lamellae, Golgi complexes, and mitochondria formed a conspicuous part of the perinuclear ooplasm. The one-cell embryos were either in syngamy or in the process of undergoing first cleavage. Positive evidence of cortical granule release and second polar bodies were detected in the perivitelline space. A block to polyspermy seemed to operate at the level of the inner zona.Dispermic and polyspermic ova had 3-16 pronuclei resembling those of monospermic ova and had sperm tails in the ooplasm. Sperm were also seen penetrating the inner zona and were occasionally found in the perivitelline space. Incomplete cortical granule release and early signs of cytoplasmic fragmentation were noted in polyspermic ova.Both normal and abnormal features of these ova are reported and compared with pronuclear structure in vivo and in vitro.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120120406
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructure of cortical granule release and zona interaction in monospermic and polyspermic human ova fertilized in vitro (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 6 (1982), S. 225-234 
    Details
    ISSN: 0148-7280
    Keywords: in vitro fertilization ; electron microscopy ; cortical reaction ; zona reaction ; monospermy ; polyspermy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Cortical granule release and interaction with the zona pellucida are reported in monospermic and polyspermic fertilized ova and early human embryos cultured in vitro.Twenty-seven preovulatory oocytes from women with tubal or idiopathic infertility were recovered by laparoscopy, after induction of follicular maturation with clomid and human chorionic gonadotropin. These were then inseminated with husband's or donor sperm, cultured for 3-72 hr, routinely fixed in glutaraldehyde/osmium and examined ultrastructurally.Evidence of cortical granule release was observed in all ova and embryos investigated and their contents were identified either at the egg surface or in the perivitelline space or interacting with the inner zona, apparently reinforcing its structure. The latter is very likely the morphological expression of the zona reaction.Delayed release was seen in certain regions of normally fertilized ova and particularly in polyspermic ova, where massive “explosions” of granules occurred. This was attributed to delayed cortical maturation. The mechanics of release were similar in both monospermic and polyspermic ova. Spontaneous dehiscence was also described in one injured unfertilized oocyte.The significance of the cortical and zona reactions as an effective block to polyspermy at the level of the inner zona, which becomes more impenetrable to supplementary sperm, is discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120060306
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