ISSN:
1572-8773
Keywords:
iron
;
siderophore
;
alcaligin
;
Bordetella pertussis
;
Bordetella bronchiseptica
;
siderophore purification
;
iron transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Hydroxamate siderophores of virulent Bordetella pertussis and Bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(S),11,18(S)-tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone, which was previously isolated from the taxonomically-related bacterial species Alcaligenes denitrificans subsp. xylosoxydans. Alcaligin purified from iron-depleted cultures of B. pertussis and B. bronchiseptica exhibited specific growth-promoting activity under iron-restricted conditions for Bordetella indicator strains, and were active in [55Fe]ferric alcaligin transport assays. Evidence suggests that several C 2-symmetric conformations of alcaligin exist simultaneously in both methanolic and aqueous solution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00144625
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