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  • Electronic Resource  (4)
  • anisotropy  (2)
  • detection  (2)
  • 1
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was used to monitor potato stems of three cultivars for the presence of the ring rot pathogen,Clavibacter michiganensis subsp.sepedonicus, during the growing season and in progeny tubers after harvest. The highest ELISA values were obtained with the highest concentration of bacteria used to inoculate seed pieces in all cultivars tested. Low ELISA values were obtained for stems and progeny tubers selected from plants grown from seed inoculated at lower bacterial concentrations. Estimates of bacterial densities in stems and progeny tubers by immunofluorescence indicated that low ELISA values were most probably caused by low bacterial numbers. It is suggested that the sensitivity of ELISA for detecting the ring rot pathogen in potato stems and progeny tubers is a function of the concentration of bacteria in individual seed pieces.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1871-4528
    Keywords: ring rot ; Solanum tuberosum L. ; ELISA ; immunofluorescence ; eggplant ; detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato stems and tubers grown in the field from seed tubers inoculated withClavibacter michiganensis subsp.sepedonicus, which causes bacterial ring rot, were tested by indirect, sandwich enzyme-linked immunosorbent assay (ELISA) in five laboratories. Correlation between values for each experimental treatment from the five laboratories was greater (r=0.86) than correlation between values for individual samples (r=0.71). When three or more laboratories obtained ELISA values of ≥0.200 for a sample, that sample was presumed to be positive. Conversely, when three or more laboratories obtained ELISA values 〈0.200, the consensus determination was regarded as negative. The percentage of stem and tuber samples that were in agreement with the consensus ELISA determination varied from 65.5 to 96.7%. Indirect immunofluorescence tests, conducted on the same samples in two laboratories, were consistent with 83.4–91.9% of the consensus ELISA determinations. Presence or absence ofC.m. sepedonicus was confirmed in some samples by an eggplant bioassay and direct isolatiion of the bacterium. The ELISA procedure was well suited for screening large numbers of samples and this study confirms it to be a promising procedure in routine indexing of seed potatoes forC.m. sepedonicus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Geotechnical and geological engineering 15 (1997), S. 47-85 
    ISSN: 1573-1529
    Keywords: Numerical modelling ; mine design ; tabular stopes ; anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Summary This paper presents a rock mechanics design methodology applicable to steeply dipping orebodies typical of many underground hardrock mines. The first stage in the design process is the characterization of the rock mass using bothin situ and laboratory data. The effects of anisotropy on rock mass behaviour are discussed with reference to laboratory and field observations. The second stage involves the use of a number of selected numerical modelling techniques to investigate ground response in the near-field rock mass surrounding the mining excavations. This study shows that the use of several numerical methods in conjunction, allowing for the advantages of each method to be maximized, provides a more comprehensive analysis of the different facets of stope design. This approach differs from those in the literature which seek to compare the different numerical methods in order to select just one method best suited for a problem. The design methodology employed emphasizes the importance of developing an understanding of ground deformation mechanisms as opposed to predicting absolute behaviour.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Geotechnical and geological engineering 15 (1997), S. 47-85 
    ISSN: 1573-1529
    Keywords: Numerical modelling ; mine design ; tabular stopes ; anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract This paper presents a rock mechanics design methodology applicable to steeply dipping orebodies typical of many underground hardrock mines. The first stage in the design process is the characterization of the rock mass using both in situ and laboratory data. The effects of anisotropy on rock mass behaviour are discussed with reference to laboratory and field observations. The second stage involves the use of a number of selected numerical modelling techniques to investigate ground response in the near-field rock mass surrounding the mining excavations. This study shows that the use of several numerical methods in conjunction, allowing for the advantages of each method to be maximized, provides a more comprehensive analysis of the different facets of stope design. This approach differs from those in the literature which seek to compare the different numerical methods in order to select just one method best suited for a problem. The design methodology employed emphasizes the importance of developing an understanding of ground deformation mechanisms as opposed to predicting absolute behaviour.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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